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RISS 인기검색어
- 검색키워드
- 저자 : Mallappa Kumara Swamy (검색결과 2 건)
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Mallappa Kumara Swamy,Pradeep S Kaushik,Subbanarasiman Balasubramanya,Maniyam Anuradha 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.1
plant. Axillary and terminal buds of these in vitro-raised seedlings formed the primary source of explants for direct organogenesis. Explants were inoculated onto MS medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BA) and kinetin (KN). The best morphogenic response was observed on MS media supplemented with 0.25 mg L-1 BA and 0.25 mg L-1 KN, which exhibited the highest regeneration frequency (84%), the maximum number of shoots/explants (63.1 ± 1.35) and shoot length (2.8 ± 1.15) within 4 weeks. Fortification of 1.0 mg L-1 GA3 enhanced the shoot elongation by 2.33 fold in 91% of shoot cluster cultures within 3 weeks. A high percent frequency of rooting (92.13%) was achieved within 15 days of shoot implantation on ½ strength MS media fortified with 100 mg L-1 activated charcoal. The rooted plantlets were successfully acclimatized with 95% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed that all the regenerated plants were genetically identical to their mother plant, suggesting an absence of detectable genetic variation in the regenerated plantlets. High performance liquid chromatography (HPLC) was done to further confirm the existence of qualitative and quantitative differences in the major secondary metabolite (camptothecin) between the mother plant and in vitro-propagated plants. The present results evidently showed comparable chemical profiles. Thus, the present protocol can be used for clonal mass propagation of true-to-type elite O. mungos plants. An efficient protocol has been established for rapid multiplication of Ophiorrhiza mungos Linn. (Rubiaceae), a potent anticancerplant. Axillary and terminal buds of these in vitro-raised seedlings formed the primary source of explants for direct organogenesis. Explants were inoculated onto MS medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BA) and kinetin (KN). The best morphogenic response was observed on MS media supplemented with 0.25 mg L-1 BA and 0.25 mg L-1 KN, which exhibited the highest regeneration frequency (84%), the maximum number of shoots/explants (63.1 ± 1.35) and shoot length (2.8 ± 1.15) within 4 weeks. Fortification of 1.0 mg L-1 GA3 enhanced the shoot elongation by 2.33 fold in 91% of shoot cluster cultures within 3 weeks. A high percent frequency of rooting (92.13%) was achieved within 15 days of shoot implantation on ½ strength MS media fortified with 100 mg L-1 activated charcoal. The rooted plantlets were successfully acclimatized with 95% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed that all the regenerated plants were genetically identical to their mother plant, suggesting an absence of detectable genetic variation in the regenerated plantlets. High performance liquid chromatography (HPLC) was done to further confirm the existence of qualitative and quantitative differences in the major secondary metabolite (camptothecin) between the mother plant and in vitro-propagated plants. The present results evidently showed comparable chemical profiles. Thus, the present protocol can be used for clonal mass propagation of true-to-type elite O. mungos plants.
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Mallappa Kumara Swamy,Sudipta Kumar Mohanty,Maniyam Anuradha 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.2
The effect of plant growth regulators and natural supplements on the morphogenetic response of Pogostemon cablin Benth. wasinvestigated. Murashige and Skoog (MS) media supplemented with 0.5 mg L-1 benzyl-6-adenine and 0.5 mg L-1 kinetin was effectivein inducing multiple shoots (63.20 ± 0.15) with an average shoot length of 5.27 ± 0.15 cm and biomass of 5.20 ± 0.10 g shoot-1. Among the natural supplements, 10% coconut water supplemented to MS media showed a better response in all the morphologicalparameters studied. The use of 10% tomato extract, 20% banana extract, 10% carrot extract, and 10% papaya extract in MS mediumhave efficiently increased multiple shoots, shoot length, and fresh weight of the shoots. The natural supplements also effectivelyincreased the chlorophyll content, total protein, and total carbohydrate content in the plant. The frequency of rooting (93%) was highestwhen shoots were implanted on strength MS media with 100 mg L-1 activated charcoal. The in vitro rooted plants were successfullyacclimatized and established in soil. Also, RAPD analysis showed no variation suggesting true-to-type nature of the micropropagatedplants. Hence, this protocol can effectively reduce the cost of in vitro multiplication of plants.
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