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박상수(Sang-Soo Park),심미령(Mi-Ryung Sim),이충기(Chung-Ki Lee) 한국경제연구학회 2017 한국경제연구 Vol.35 No.3
2007년부터 시행되고 있는 한국의 주택연금제도는 정부가 보증하는 역모기지 프로그램이다. 한국주택금융공사가 매년 실시하고 있는 연간 설문 자료를 이용하여 미래에 주택연금을 이용할 의향이 있는 가구들에 대해 주택연금에 대한 수요를 추정하였다. 본 논문에서는 이충기․박상수(2014)의 연구를 확장하여 미래 기대와 코호트 효과를 고려하여 순서형 로짓 모형을 설정하였다. 설문에서 진술된 가구의 의향이 미래에 대한 기대에 근거하고 있다는 점을 적절하게 평가하기 위해 몇 가지의 가정을 바탕으로 미래의 기대형성 과정을 계량경제학적으로 모형화하여 주택연금 수요를 예측하고자 하였다. 주택연금에 대한 수요 추정 결과에 따르면, 주택연금 프로그램에 대해 후세대들이 보다 덜 긍정적인 경향이 나타난 반면 저소득 계층은 보다 긍정적으로 나타났다. The J ooTaekYeonKeum is the government guaranteed reverse mortgage program that Korean Government has been implementing since 2007. We extended Lee and Park’s(2014) demand estimation for the reverse mortgage loans in Korea using the yearly survey data, conducted by Korea Housing Financing Corporation, for household’s intention to use J ooTaekYeonKeum in the future. In order to appropriately appreciate the fact that the stated intentions in the survey were based on households’ expectations for their future, we adopted an econometric procedure that incorporated expectations explicitly as well as cohort effects. Our findings include: demands for reverse mortgage loans are estimated similar to that of Lee and Park(2014); later generations tend to be less positive for having the reverse mortgage program; lower income groups are more positive for the program.
Production of Transgenic Male Piglet as Research Model for Alzheimer's Disease
Mi-Ryung Park,Kyong-Woon Kim,Jae-Seok Woo,Seongsoo Hwang,In-Sul Hwang1,Tae-Uk Kwak,Ji-Hyun Lim,Se-Pil Park 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Alzheimer’s disease (AD) is a progressive neurodegenerative disease associated with memory loss and cognitive impairments. An AD transgenic (Tg) pig model would be useful for preclinical testing of therapeutic agents. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the human AD related genes (APP, APPswedish, Presenilin 1, and Tau). Transgenic embryos were generated by SCNT of from ear fibroblasts expressing AD genes. A total of 1808 (average 258) SCNT embryos were transferred into 7 recipients. Pregnancy was successfully maintained in one recipient. We obtained 1 cloned male piglet from a surrogate gilts and the weight of piglet was 935 g. But, the male piglet died two days after birth. The piglet expressed AD related genes by PCR and western blot analysis. Transgenes were expressed in multiple tissues, and at especially high levels in brain. AD Tg pig might be very useful for studying the disease and for testing new therapeutics in preclinical studies of human AD.
Low Expression of MFN1 Causes Mitochondrial Dysfunction and Apoptosis in Porcine SCNT Embryos
Mi-Ryung Park,Kyong-Woon Kim,Seongsoo Hwang,In-Sul Hwang,Tae-Uk Kwak,Ji-Hyun Lim,Jae-Seok Woo 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
The various functions of mitochondria are essential for the transition from oocyte to embryo and for early embryonic development up to the blastocyst stage. Mitofusin 1 (MFN1) is the main mediator of mitochondrial fusion and homeostasis. Mfn1 proteins are located in the outer mitochondrial membrane. The mitochondrial membrane potential is a central indicator of cellular viability that reflects indicators of metabolic activity such as oxidative phosphorylation and the electron transport process. We investigated the MFN1 expression level in porcine SCNT blastocysts. The results showed that the MFN1, mitochondrial membrane potential and ATP levels were significantly decreased in the SCNT blastocysts. Whereas Bad, Bax, Caspase-3 and Caspase-9 levels were significantly increased compared with the parthenogenetic blastocysts. This study suggests that downregulation of MFN1 could affect the blastocyst development of porcine SCNT embryos through mitochondrial function and cellular apoptosis.
Mi-Ryung Park,Jae Gyu Yoo,Chang-Gi Hur,Bo-Woong Sim,Myunghoo Kim,Jakyeom Seo,Byeong-Woo Kim,Byung-Wook Cho,Teak-Soon Shin,Seong-Keun Cho 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.3
This study investigated the effect of variation in the number of somaticcell- cloned embryos and their developmental stage at transfer on pregnancy, as well as the influence of the estrus status of recipient pigs on in vivo development of cloned porcine embryos after embryo transfer. For somatic cell nuclear transfer (SCNT), fibroblast cells were obtained from a male porcine fetus. Recipient oocytes were collected from prepubertal gilts at a local abattoir and then cultured. After SCNT, reconstructed embryos of different numbers and developmental stages were transferred into recipient pigs. The developmental stage of the cloned embryos and the number of transferred embryos per surrogate showed no significant differences in terms of the resulting cloning efficiency. However, the pregnancy rate improved gradually as the number of transferred cloned embryos was increased from 100- 150 or 151-200 to 201-300 per recipient. In pre-, peri-, and post-ovulation stages, pregnancy rates of 28.6%, 41.8%, and 67.6% and 16, 52, and 74 offspring were recorded, respectively. The number of cloned embryos and estrus status of the recipient pig at the time of transfer of the cloned embryo affect the efficiency of pig production; therefore, these variables should be particularly considered in order to increase the efficiency of somatic cell pig cloning.
Production of Human TPA Transgenic Pigs by Somatic Cell Nuclear Transfer
Mi-Ryung Park,Kyong-Woon Kim,In-Sul Hwang,JeongHee Yun,Tae-Uk Kwak,Ju-Young Lee,Namwoong Hyung,Ji-Hyun Lim,Sang-Hyoun Park,Seongsoo Hwang 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Tissue-type plasminogen activator, a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots. The purpose of this study was to produce a tPA transgenic pig by somatic cell nuclear transfer. The human tPA (htPA) genes were transfected into porcine ear fibroblast cells derived from a female piglet and established two transgenic cell lines (Tg) with normal karyotypes. Tg line #3 and #4, which expressed high levels of the transgenes. Blastocyst rates of SCNT embryos derived from Tg lines were significantly lower than those of Non-Tg. A total of 2799 SCNT embryos were transferred to 9 surrogates. Seven offspring were delivered, and the production efficiency was 1.0~1.3% (3/324 and 4/304). All offspring exhibited htPA genes. These piglets revealed by microsatellite testing to be genetically identical to Tg cell lines, but not to the surrogate. Our goal is to establish a htPA transgenic pig for studying biopharmaceutical production.
Effects of CD26 in Parthenogenetically Activated Porcine Embryos
Park, Mi-Ryung,Im, Ji-Hyun,Chung, Hak-Jae,Kim, Kyong-Woon,Byun, Sung June,Hwang, Seongsoo,Im, Gi-Sun The Korean Society of Embryo Transfer 2016 한국동물생명공학회지 Vol.31 No.4
CD26, also known as Dipeptidyl peptidase IV (DPP-4), is a cell surface glycoprotein that belongs to the serine protease family and has wide spread organ distribution throughout the body. CD26 was previously characterized in immune cells but also has important metabolic functions which are not yet fully understood. Thus, we investigated the effect of CD26 in porcine parthenogenetic embryos. We attempted CD26 downregulation of porcine embryos by siRNA, and evaluated CD26 suppression of developmental competencies. Although the porcine embryos injected with CD26 siRNA were able to develop to the early stage, these embryos were decreased to form blastocysts. Our results indicated that CD26 is one of factors for the regulation of development of porcine embryos.
Chitosan nanoparticles cause pre- and postimplantation embryo complications in mice.
Park, Mi-Ryung,Gurunathan, Sangiliyandi,Choi, Yun-Jung,Kwon, Deug-Nam,Han, Jae-Woong,Cho, Ssang-Goo,Park, Chankyu,Seo, Han Geuk,Kim, Jin-Hoi Society for the Study of Reproduction [etc.] 2013 BIOLOGY OF REPRODUCTION Vol.88 No.4
<P>Embryo development is a complex and tightly controlled process. Nanoparticle injury can affect normal development and lead to malformation or miscarriage of the embryo. However, the risk that these nanoparticles may pose to reproduction is not clear. In this study, chitosan nanoparticles (CSNP) of near uniform size, in the range of 100 nm, were synthesized and confirmed by a particle size analyzer and transmission electron microscopy. Morulae-stage embryo exposure to CSNP during in vitro culture caused blastocyst complications that had either no cavity or a small cavity. Furthermore, CSNP-treated embryos showed lower expression of not only trophectoderm-associated genes but also pluripotent marker genes. When blastocysts developed in both media with and without CSNP were transferred to recipients, the percentage of blastocysts resulting in viable pups was significantly reduced. These detrimental effects are linked to the reduction of total cell numbers, enhanced apoptosis, and abnormal blastocoels forming at the blastocyst stage, indicating that CSNP treatment might have long-term adverse biological effects in view of pregnancy outcome.</P>