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- 저자 : M. Barta (검색결과 2 건)
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Bacterial Logic Devices Reveal Unexpected Behavior of Frameshift Suppressor tRNAs
Sawyer, Eric M.,Barta, Cody,Clemente, Romina,Conn, Michel,Davis, Clif,Doyle, Catherine,Gearing, Mary,Ho-Shing, Olivia,Mooney, Alyndria,Morton, Jerrad,Punjabi, Shamita,Schnoor, Ashley,Sun, Siya,Suresh, Korean Society for Bioinformatics 2012 Interdisciplinary Bio Central (IBC) Vol.4 No.3
Introduction: We investigated frameshift suppressor tRNAs previously reported to use five-base anticodon-codon interactions in order to provide a collection of frameshift suppressor tRNAs to the synthetic biology community and to develop modular frameshift suppressor logic devices for use in synthetic biology applications. Results and Discussion: We adapted eleven previously described frameshift suppressor tRNAs to the BioBrick cloning format, and built three genetic logic circuits to detect frameshift suppression. The three circuits employed three different mechanisms: direct frameshift suppression of reporter gene mutations, frameshift suppression leading to positive feedback via quorum sensing, and enzymatic amplification of frameshift suppression signals. In the course of testing frameshift suppressor logic, we uncovered unexpected behavior in the frameshift suppressor tRNAs. The results led us to posit a four-base binding hypothesis for the frameshift suppressor tRNA interactions with mRNA as an alternative to the published five-base binding model. Conclusion and Prospects: The published five-base anticodon/codon rule explained only 17 of the 58 frameshift suppression experiments we conducted. Our deduced four-base binding rule successfully explained 56 out of our 58 frameshift suppression results. In the process of applying biological knowledge about frameshift suppressor tRNAs to the engineering application of frameshift suppressor logic, we discovered new biological knowledge. This knowledge leads to a redesign of the original engineering application and encourages new ones. Our study reinforces the concept that synthetic biology is often a winding path from science to engineering and back again; scientific investigations spark engineering applications, the implementation of which suggests new scientific investigations.
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B. Jansky,Z. Turzik,E. Novak,M. Svadlenkova,M. Barta,L. A. Trykov,A. I. Blokhin 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
The leakage neutron and gamma spectra measurements have been done on benchmark spherical assembly-nickel sphere with diameter of 50 cm. The Cf-252 neutron sources with different emissions were placed into the centre of nickel sphere. The proton recoil method was used for neutron spectra measurement using stilbene crystals and hydrogen proportional counters. The neutron energy range of spectrometer was from 0.02 to 17 MeV. The gamma pulse shape discrimination method has been applied in stilbene measurements. The gamma energy range of spectrometer was from 0.1 to 10 MeV. The fine structure of gamma spectrum was measured by HPGe spectrometer. The experimental data were compared to results of transport calculations based on different evaluated nuclear data libraries (ENDF/B-VII.0, JENDL-3.3, JEFF-3.1.1, TENDL-2009). The continuous energy Monte Carlo transport calculation code MCNP-4C was employed for the calculations. Main observed differences between experiments and transport calculations are discussed.
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