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- 저자 : Luo Sufeng (검색결과 2 건)
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Luo Juan,Bai Luyan,Tao Jun,Wen Yu,Li Mingke,Zhu Yunzhen,Luo Sufeng,Pu Guangyu,Ma Lanqing 한국유전학회 2021 Genes & Genomics Vol.43 No.10
Background Vacuolating cytotoxin (VacA) is an important virulence factor of Helicobacter pylori (H. pylori). It was previously believed that VacA can trigger the cascade of apoptosis on mitochondria to lead to cell apoptosis. Recently, it was found that VacA can induce autophagy. However, the molecular mechanism by which VacA induces autophagy is largely unknown. Objective We aimed to explore the molecular mechanism of autophagy induced by H. pylori in gastric cancer cells and the efect of autophagy on the survival of gastric cancer cells. Methods The autophagy of human gastric cancer cell line SGC7901 was detected by Western blot and RT-PCR in the treatment of VacA protein of H. pylori. The relationship between autophagy and reactive oxygen species (ROS) in the proliferation of gastric cancer cells were studied by gene expression silences (siRNA) and CM-H2DCFDA (DCF) staining. Results The results showed that VacA protein secreted by H. pylori in the supernatant stimulated autophagy in SGC7901 cells. After VacA protein treatment, the mRNA expressions of BECN1, ATG7 and PIK3C3, were up-regulated. ATG7 silencing by siRNA inhibited VacA-induced autophagy. Furthermore, our data demonstrated that VacA protein increased ROS levels. Addition of the antioxidant N-acetyl-l-cysteine (NAC) suppressed the levels of ROS, leading to inhibition of autophagy. Conclusions H. pylori VacA is a key toxin that induces autophagy by increased ROS levels. And our fndings demonstrated that VacA signifcantly inhibited proliferation in SGC7901 cells.
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Su Feng,Weicai Zeng,Fan Luo,Jian Zhao,Zhirong Yang,Qun Sun 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.1
Pine needle (Pinus massoniana Lamb.) of large quantity in China and health benefit makes its application on pharmaceutical and food industry in high demand. The chemical composition of pine needle aqueous extract (PNAE) analyzed by gas chromatograph-mass spectrometry revealed that among more than 10 compounds in PNAE,organic acids were over 76.92%, with acetic acid being 25.20%, hexadecanoic acid 18.19%, and 2-methoxy-4-vinylphenol 16.44%. Ultra-performance liquid chromatography-mass spectrometry disclosed other 5 short chain organic acids, including citric acid, succinic acid, malonic acid, malic acid, and oxalic acid. The antibacterial activity of PNAE on common spoilages and pathogenic bacteria showed that the growth of Bacillus subtilis, Staphylococcus aureus, Bacillus cereus, Micrococcus luteus, Escherichia coli, and Proteus vulgaris were inhibited significantly, with minimum inhibitory concentrations and minimum bactericidal concentrations being 3.8-15 and 7.5-30 mg/mL, respectively. Our findings suggested that pine needles with effective and safe antibacterial components possess the potential to be developed into efficacious natural antiseptic products for food disinfection and medical purpose.
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