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      • A Basic Study on Regeneration System using Plasma for Diesel Particulate Filter

        Ruiqiang Liu(류서강),Jae-Ou Chae(채재우),Yong-Hwan Kwak(곽용환),Xinhong Chen(진신홍) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6

        Diesel Particulate Filter (DPF) is the most widely used method to control particulate emissions of diesel engine these days. However, DPF needs periodical regeneration because of soot deposition. Electric discharge plasma, with its strong oxidation characteristics can be utilized in the regeneration process even at lower exhaust temperatures, and the present study also confirmed that the regeneration of DPF was realized when the temperature was elevated from 200℃. Plasma oxidizes NO in the exhaust to NO<SUB>2</SUB> and NO<SUB>2</SUB> in turn oxidizes soot in presence of oxygen to release CO and CO<SUB>2</SUB>. The advantage with plasma regeneration is it proceeds at a lower temperature at which no damage to DPF can happen. Initial experiments were conducted on optimizing NO oxidation to NO<SUB>2</SUB> using plasma reactor. In the next step, regeneration of DPF was carried out. Different parameters such as applied high voltage and gas temperature are studied.

      • KCI등재

        Preventive effect of Lactobacillus plantarum HFY09 on HCl/ethanol-induced gastric injury in mice

        Li Fang,Sun Hailan,Ran Guangjun,Liu Xinhong,Yi Ruokun,Tan Fang,Zhao Xin,Liu Huazhi 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.5

        The aim of this study was to investigate the effect of Lactobacillus plantarum HFY09 on gastric injury induced by HCl/ ethanol in Kunming mice. The results showed that HFY09-H inhibited any increases in gastric juice volume, maintained the normal pH value of gastric acid, and reduced the damage caused to the gastric mucosa and gastric wall, the inhibition rate on the injury area reaches 63.70%. Compared with the negative control group, HFY09 increased the levels of serum somatostatin (SS) and vasoactive intestinal peptide (VIP), and also decreased the levels of substance P (SP), endothelin-1 (ET-1), interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). In addition, real time fluorescent quantitative PCR (Q-PCR) also confirmed that high-dose HFY09 ( 109 CFU/kg/ day) upregulated the mRNA expression of copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS), and downregulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). At the same time, the results of the HFY09 treatment group were similar to those of the ranitidine treatment group. These results indicate that HFY09 can prevent gastric injury induced by HCl/ethanol in vivo. Therefore, HFY09 may play a potential role in the treatment of gastric diseases.

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        A Lectin Receptor Kinase Positively Regulates ABA Response During Seed Germination and Is Involved in Salt and Osmotic Stress Response

        Keqin Deng,Qiming Wang,Jianxin Zeng,Xinhong Guo,Xiaoying Zhao,Dongying Tang,Xuanming Liu 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6

        Lectin receptor-like kinases (LecRK) are widespread in higher plants; however, little is known about their physiological roles. In this study, At1g70130 (designated LecRK-b2), an Arabidopsis LecRK gene, has been investigated. LecRK-b2 was predominantly expressed during seed germination, and its expression was ceased following germination. The expression of LecRK-b2 was induced by abscisic acid (ABA), salt, and osmotic stress. LecRK-b2 lossof- function mutation slightly reduced the ABA sensitivity during seed germination, and this reduced sensitivity was demonstrated not due to lower ABA accumulation level in the seeds. Dual-luciferase transient expression assay confirmed that the transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3) could activate the luciferase under driving of LecRK-b2 promoter. LecRK-b2 transcription level was found to be down-regulated in abi3 during seed germination. Furthermore, LecRK-b2 loss-of-function mutation reduced the salt and osmotic sensitivity during early development stage of Arabidopsis. Taken together, these results suggest that LecRK-b2 functions as a positive regulator of the ABA response during the seed germination and is involved in salt and osmotic stress response in the early development stage.

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        Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions

        Lingyu Li,Haomeng Yang,Weibo Ren,Bei Liu,Dongmei Cheng,Xinhong Wu,Jirui Gong,Lianwei Peng,Fang Huang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4

        Sheepgrass [Leymus chinensis (Trin.) Tzvel] is a valuble forage plant highly significant to regional grassland productivity of Euro-Asia steppes. Although effects of environmental stress including drought have been studied, impact of nutrient deficiency in particular phosphate (Pi), one of the essential macronutrient, is not reported. Here, we investigated low-Pi effect on its photosynthetic apparatus via physiological and biochemical analysis. We show that PSII activity was significantly reduced based on chlorophyll fluorescence measurements. We observed decreased amount of core proteins of PSII by immunoblot analysis. Further analysis of thylakoid membranes using 2D-BN/SDS-PAGE and immunoblot detection demonstrated that the amount of PSII complexes was closely correlated with the Pi levels within the range. Together with reduced number of thylakoid grana stackings observed, we suggest that the maintenance of PSII is impaired under Pi-limited condition. Moreover, enzyme activity assays revealed that the activity of several ROS scavenger enzymes was stimulated by low-Pi treatment. Based on these experimental results we conclude that PSII is the component of photosynthetic apparatus most sensitive to Pi supply and the enhanced anti-ROS activity is mainly subjective to protection of PSII against low Pi-induced photo-oxidative stress in the organism.

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