http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Liangjiang Wang (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Identification of olfactory receptor genes in the Japanese grenadier anchovy Coilia nasus
Guoli Zhu,Liangjiang Wang,Wenqiao Tang,Xiaomei Wang,Cong Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.5
Olfaction is essential for fish to detect odorant elements in the environment and plays a critical role in navigating, locating food and detecting predators. Olfactory function is produced by the olfactory transduction pathway and is activated by olfactory receptors (ORs) through the binding of odorant elements. Recently, four types of olfactory receptors have been identified in vertebrate olfactory epithelium, including main odorant receptors (MORs), vomeronasal type receptors (VRs), trace-amine associated receptors (TAARs) and formyl peptide receptors (FPRs). It has been hypothesized that migratory fish, which have the ability to perform spawning migration, use olfactory cues to return to natal rivers. Therefore, obtaining OR genes from migratory fish will provide a resource for the study of molecular mechanisms that underlie fish spawning migration behaviors. Previous studies of OR genes have mainly focused on genomic data, however little information has been gained at the transcript level. In this study, we identified the OR genes of an economically important commercial fish Coilia nasus through searching for olfactory epithelium transcriptomes. A total of 142 candidate MOR, 52 V2R/OlfC, 32 TAAR and two FPR putative genes were identified. In addition, through genomic analysis we identified several MOR genes containing introns, which is unusual for vertebrate MOR genes. The transcriptomescale mining strategy proved to be fruitful in identifying large sets of OR genes from species whose genome information is unavailable. Our findings lay the foundation for further research into the possible molecular mechanisms underlying the spawning migration behavior in C. nasus.
-
Proteomics Analysis of Rice Proteins Up-regulated in Response to Bacterial Leaf Streak Disease
Dongxiao Li,Liangjiang Wang,Shaolei Teng,Guoguang Zhang,Lijia Guo,Qian Mao,Wei Wang,Min Li,Liang Chen 한국식물학회 2012 Journal of Plant Biology Vol.55 No.4
Bacterial leaf streak (BLS), caused by the pathogen Xanthomonas campestris pv. Oryzicola, is a major rice disease in tropical and subtropical regions of Asia. Rice proteins responsive to BLS are still not well characterized. We took a proteomics approach to identify the proteins that are up-regulated in rice leaves after infection. Approximately 1,500 protein spots were detected on each 2-D gel after silver-staining; those with increased protein levels were selected for MALDI-TOF-MS analysis. We identified 32 up-regulated proteins that might be involved in disease resistance signal transduction, pathogenesis, and regulation of cell metabolism. By using publicly available microarray data, we determined the mRNA transcripts of 23 proteins expressed in the leaves. Seven genes were analyzed by northern blots, which demonstrated that transcript levels were increased after bacterial infection. Our findings help elucidate the molecular mechanisms underlying BLS and provide a solid foundation for further research on the functions of relevant genes.
-
Min Li,Lijia Guo,Chiming Guo,Liangjiang Wang,Liang Chen 한국식물학회 2016 Journal of Plant Biology Vol.59 No.1
Salt stress has adverse effects on the growth and production of rice crops. In this study, we isolated and characterized SIDP361, which encodes a DUF1644 family protein. This gene was expressed in various rice tissues and was induced by high salt (200 mM NaCl), dehydration, and abscisic acid (100 µM ABA) treatments. Stable expression of SIDP361-GFP in rice cells suggested that SIDP361 is a cytoplasmic protein. When compared with the untransformed wild-type (WT) control, transgenic plants over-expressing SIDP361 exhibited significantly improved tolerance to salt stress at both the seedling and heading stages. Under salinity conditions, the transgenics also had elevated amounts of free proline. Moreover, transcript levels for genes encoding proline synthetase enzymes were significantly higher in transformants than in the WT. The transgenic lines were also hypersensitive to exogenous ABA. Quantitative real-time PCR analysis showed that transcription of several stressrelated genes was greater in SIDP361-overexpressing plants than in the WT under both normal and salt-stressed conditions. These results demonstrate that SIDP361 has high potential as a tool for genetically improving salt tolerance in rice.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
