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Li Shengyun,Chen Song,Dong Shuanglin,Zhang Min,Deng Zhongyuan,Ni Xinzhi,Huang Jinyong,Li Xianchun 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3
The enrichment of transposable elements (TEs) within allelochemical- and insecticide-metabolizing P450 alleles in Helicoverpa zea enables these P450s to gain TE-introduced adaptive variations otherwise not readily available to cope with the ever-changing and diverse xenobiotic stress factors in varying cropping systems. The critical role of each TE-inserted P450 allele depends on whether the inserted P450 allele is more adaptive than its TE-free counterpart or not. Previous study has reported a HzSINE1-inserted CYP321A2 allele in a laboratory strain of H. zea reared with xenobiotic-free artificial diets. Here we show that the HzSINE1-inserted CYP321A2 allele transcribes into two HzSINE1 sequence-containing mutant mRNA isoforms of different length that encode an identical C terminus-truncated and heme-binding region deleted non-functional P450. Nonetheless, HzSINE1 insertion does not disrupt the regulatory functional aspect of CYP321A2 since this allele is constitutively expressed and highly inducible by the allelochemicals xanthotoxin, quercetin and chlorogenic acid. Furthermore, while the HzSINE1-inserted CYP321A2 allele is fixed in the laboratory strain, the insertion is purged in the bifenthrin-resistant strain and the Georgia field population of H. zea. To sum up, the HzSINE1-inserted CYP321A2 allele represents an allelochemical-inducible non-functional P450 allele that is selected against in the field populations frequently encountering toxic plant allelochemicals and synthetic insecticides. However, such an insertion can reach fixation under the xenobiotic-free laboratory rearing conditions most likely due to random genetic drift across multiple generations.
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Ruyan Liu,Yin Chi,Shengyun Chen,Qinghui Jiang,Xiaoyu Meng,Kun Wu,Shengjia Li 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.4
Pervious concrete has been widely used in parking lots and other lightweight streets. Performances of pervious concrete are strongly dependent on its pore structure characteristics. This paper investigates the relationship among porosity, permeability, compressive strength, durability and the pore structure characteristics of pervious concrete. The influences of basalt fiber and fine aggregate on the pervious concrete are also discussed. Pervious concrete specimens with different pore structures were prepared by combining basalt aggregate with size of 5-10 mm and 10-15 mm at different proportion. The pore structure characteristics such as area porosity, pore sizes, and pore distribution of pervious concrete were obtained and analyzed using image analysis method. Experimental results showed that the pore structure characteristics of pervious concrete have effects on its mechanical and durability behaviors. Homogeneous pore distribution and large distances between pores increase the compressive strength and improve freeze-thaw durability of pervious concrete. Due to the difference in the internal pore structure of pervious concretes, specimens with similar volumetric porosities had different permeability coefficients. A series of numerical models were regenerated through a MATLAB code using pore structural parameters derived from the image analysis method, then the numerical models were fed to ANSYS for meshing and further simulation. The comparison of the simulation and experimental results showed close accuracy, which verified the applied simulation analysis method based on image analysis technology.
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