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LncRNA PVT1 upregulates FBN1 by sponging miR-30b-5p to aggravate pulpitis
Li Yuanyuan,Li Shuangshuang,Li Ruijing,Xu Huilin 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.2
Background Pulpitis is a disease mainly caused by bacterial infection. Long noncoding RNAs (lncRNAs) infl uence the advancement of pulpitis. In this paper, we reconnoitered the characters of lncRNA plasmacytoma variant translocation 1 (PVT1) in pulpitis. Objective PVT1, microRNA-30b-5p (miR-30b-5p) and fi brillin 1 (FBN1) levels were identifi ed by qRT-PCR and western blot. In addition, the cell functions were scrutinized. Additionally, the link between miR-30b-5p and PVT1 or FBN1 was identifi ed by dual-luciferase reporter assay. Result The contents of PVT1 and FBN1 were augmented, but the miR-30b-5p level was declined in pulpitis. Knockdown of PVT1 enhanced cell proliferation and cell vitality, whereas inhibited cell infl ammatory reaction in HDPFs under LPS stimulation. In mechanism, PVT1 acted as a miR-30b-5p sponge to adjust the content of FBN1. Moreover, miR-30b-5p mediated LPS-mediated repression of proliferation and promotion of infl ammatory reaction HDPFs by FBN1. Conclusion PVT1 expedited the improvement of pulpitis via miR-30b-5p/FBN1.
Xun-an Ning,Chongyang Yang,Yujie Wang,Zuoyi Yang,Jingyu Wang,Ruijing Li 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4
A strict aerobic Acinetobacter baumanniiYNWH 226, isolated from an activated sludge reactortreating textile wastewater, was able to grow on Congo redas the sole carbon source under aerobic conditions. Thedecolorization and TOC reduction efficiency were 99.1 and93.72%, respectively. The effects of the Congo redconcentration were studied. The environmental factors (i.e.,pH, temperature and agitation speed) on the biodegradationof Congo red in aqueous phase were studied and evaluatedusing response surface methodology. The results indicatedthat when the Congo red concentration was 100 mg/L, theoptimal decolorization conditions were as follows: 37°C,pH 7.0 and 180 rpm. The single A. baumannii YNWH 226was able to form aromatic amines by reductive breakage ofthe azo bond and then oxidize them into non-toxicmetabolites.