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- 저자 : Leiping Hou (검색결과 2 건)
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Identification of pepper genes involved in the response to CO 2 enrichment using RNA-Seq analysis
Jing Zhang,Rui Bai,Mengya Shang,Xiaoyong Xu,Hongxia Song,Shaowen Zheng,Leiping Hou,Meilan Li,Guoming Xing 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1
Pepper is widely cultivated, and the application of CO 2 promotes photosynthesis and increases its yield. However, the molecularmechanisms underlying this are still unclear. In this study, the photosynthetic correlation indexes under elevated CO 2and control conditions were compared. The application of CO 2 increased the photosynthetic capacity of pepper. Moreover,RNA-Seq analysis was used to identify genes that were diff erentially expressed between pepper leaves grown in CO 2 -enrichedconditions and those grown in control conditions. The 149 diff erentially expressed genes (DEGs) were found to be involvedin photosynthesis and other metabolic processes. According to GO signifi cant enrichment analysis, the proteins encodedby the DEGs were mainly found to be located in the chloroplast, the chloroplast matrix, and the apoplast. According toKEGG signifi cant enrichment analysis, the DEGs were found to be involved in glutathione metabolism; starch and sucrosemetabolism; and stilbenoid, diarylheptanoid, gingerol, fl avonoid, and phenylpropanoid biosynthesis. The DEGs were alsoinvolved in the pentose phosphate pathway, carbon metabolism, and porphyrin and chlorophyll metabolism. Based on theGO annotation and the KEGG database analysis, ten of the DEGs identifi ed were suggested to be involved in photosynthesisand related processes; these genes were predicted to have roles in carbohydrate, soluble sugar, and glutathione metabolism,and in raffi nose, cysteine, nucleotide, and ABA biosynthesis. These DEGs are involved in the pentose phosphate pathwayand tricarboxylic acid cycle of carbon assimilation during photosynthesis. One of the DEGs was also found to be involved inchlorophyll biosynthesis. These results lay the foundation for further investigation of the molecular mechanisms and genesinvolved in the response to CO 2 enrichment in peppers.
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Xianhui Qi,Brad Townsley,José Antonio Aguilar-Martínez,Lihui Yin,Xingying Gao,Leiping Hou,Meiying Gao,Meilan Li 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.6
Flowering is critical to the growth and development of plants, and LFY gene homologues play a major role in flowering initiation. To understand the genetic and molecular mechanisms underlying floral initiation and development in Brassica rapa subsp. pekinensis, BrpLFY, a homologue of LFY, was cloned using RT-PCR. Sequence analysis showed that the cDNA sequence of BrpLFY is 1,341 bp in length, with an ORF of 1,245 bp encoding a predicted protein of 415 amino acids. The predicted protein showed a high degree of identity with LFY homologues from other angiosperm species. Real-time PCR analysis showed that BrpLFY mRNA was detected in all tissues during plant development from the vegetative state to fully differentiated flowers, and its expression was highest in the cotyledon and lowest in the root. BrpLFY expression in the shoot apex increased gradually during vegetative growth and increased dramatically at stage 1 of flower bud differentiation. The relative expression peaked at stage 5 and then decreased in later stages. Moreover, the trend in BrpLFY expression level change in the shoot apex was similar regardless of variety or vernalization method. The relative expression of BrpLFY in leaves gradually decreased with leaf development. We overexpressed the gene in Arabidopsis thaliana using the floral dip method, and examined flowering time in wild-type and transgenic plants. Overexpression of BrpLFY specifically caused early flowering; the transgenic plants flowered 10-14 d earlier than did wild-type plants, and leaf number decreased by 0.5-1 when the plants bolted. Real-time PCR analysis showed that the expression of BrpLFY in transgenic Arabidopsis was higher than in wild-type plants. These results indicate that BrpLFY plays a role in promoting flowering in Chinese cabbage.
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