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- 저자 : LeeChang Seung (검색결과 1 건)
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Lee, Chang‐,Ro,Cho, Seung‐,Hyon,Kim, Hyun‐,Jin,Kim, Miri,Peterkofsky, Alan,Seok, Yeong‐,Jae Blackwell Publishing Ltd 2010 Molecular microbiology Vol.78 No.6
<P><B>Summary</B></P><P>An <I>Escherichia coli</I> mutant devoid of enzyme IIA<SUP>Ntr</SUP> (EIIA<SUP>Ntr</SUP>) of the nitrogen PTS is extremely sensitive to leucine‐containing peptides due to decreased expression of acetohydroxy acid synthase. This decreased expression is due to defective potassium homeostasis. We further elucidate here the mechanism for regulation of gene expression by the intracellular level of K<SUP>+</SUP>. The leucine hypersensitivity of a <I>ptsN</I> (encoding EIIA<SUP>Ntr</SUP>) mutant was suppressed by deleting <I>rpoS</I>, encoding the stationary phase σ factor. Despite intracellular levels of sigma factors comparable to the wild‐type strain, most of the genes downregulated in a <I>ptsN</I> mutant are controlled by σ<SUP>70</SUP>, while all the upregulated genes are controlled by σ<SUP>S</SUP>, implying that the balance of sigma activities is modified by <I>ptsN</I> deletion. This change of sigma factor activities in the deletion mutant was found to be due to increased levels of K<SUP>+</SUP>. <I>In vitro</I> transcription assays demonstrated that a σ<SUP>70</SUP> controlled gene and a σ<SUP>S</SUP> controlled gene were differentially affected by potassium concentration. Biochemical studies revealed that K<SUP>+</SUP> is responsible for sigma factor competition by differentially influencing the binding of σ<SUP>70</SUP> and σ<SUP>S</SUP> to core RNA polymerase. Taken together, the data suggest that EIIA<SUP>Ntr</SUP> controls sigma factor selectivity by regulating the intracellular K<SUP>+</SUP> level.</P>
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