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- 저자 : LeTourneau Melissa K. (검색결과 1 건)
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Wang Xianguo,Yao Xiaolu,Zhao Ahui,Yang Mingming,Zhao Wanchun,LeTourneau Melissa K.,Dong Jian,Gao Xiang 한국유전학회 2021 Genes & Genomics Vol.43 No.10
Background Phosphoinositide-specifc phospholipase C proteins mediate environmental stress responses in many plants. However, the potential of PI-PLC genes involved with abiotic stress tolerance in wheat remains un-explored. Objective To study TaPLC1 genetic relation with wheat drought and heat resistance. Methods The seedlings were treated with PI-PLC inhibitor U73122 at the single leaf stage. The seedlings were treated with drought and heat stress at the two leaf stage, and some physiological indexes and the expression profle of TaPLC1 gene were determined. And the TaPLC1 overexpression vector was transferred to Arabidopsis and selected to T3 generation for drought and heat stress treatment. Results After 4 h of drought and heat stress, the SOD activity, MDA and soluble sugar content of the two cultivars with inhibitor were higher than those without inhibitor, the chlorophyll content decreased. CS seedlings showed signifcant wilting phenomenon, and TAM107 showed slight wilting. After the elimination of drought and heat stress, all seedling wilting gradually recovered, while the leaf tips of the two varieties treated with inhibitors began to wilt and turn yellow, which was more signifcant 5 days after the drought and heat stress, while the degree of spring wilting and yellow in CS was earlier than that in TAM107. The expression patterns of TaPLC1 gene were diferent in the two cultivars, but the expression levels reached the maximum at 30 min of heat stress. The change of TaPLC1 expression in TAM107 without inhibitor treatment was signifcantly greater than that in CS. The expression level of TaPLC1 in the two cultivars under stress was signifcantly diferent between the two cultivars treated with inhibitor and untreated, and was lower than that of the normal plants under normal conditions. These results indicated that inhibition of TaPLC1 gene expression could enhance the sensitivity of seedlings to stress. In Arabidopsis, the root lengths of transgenic and wild-type seedlings were shortened after drought stress treatment, but the root lengths of transgenic plants decreased slightly. And the expression of TaPLC1 gene was signifcantly increased after drought and heat stress. This indicated that overexpression of TaPLC1 improved drought resistance of Arabidopsis. Conclusions The results of this study suggest that TaPLC1 may be involved in the regulation mechanism of drought and heat stress in wheat.
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