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- 저자 : Lalman, Jerald A. (검색결과 1 건)
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Elucidating acetogenic H<sub>2</sub> consumption in dark fermentation using flux balance analysis
Lalman, Jerald A.,Chaganti, Subba Rao,Moon, Chungman,Kim, Dong-Hoon Elsevier 2013 Bioresource technology Vol.146 No.-
<P><B>Abstract</B></P> <P>In this study, a flux balance analysis (FBA) was adopted to estimate the activity of acetogenic H<SUB>2</SUB>-consuming reaction. Experimental data at different substrate concentrations of 10, 20, and 30g COD/L showing the lowest, medium, and highest H<SUB>2</SUB> yields, respectively, were used in the FBA to calculate the fluxes. It was interesting to note that the hydrogenase activity based on R12 (2Fd<SUP>+</SUP> +2H<SUP>+</SUP> →2Fd<SUP>2+</SUP> +H<SUB>2</SUB>, ferredoxin (Fd)) flux was most active at 10g COD/L. The flux of R17 (4H<SUB>2</SUB> +2CO<SUB>2</SUB> →CH<SUB>3</SUB>COOH), a mechanism for reutilizing produced H<SUB>2</SUB>, increased in steps of 0.030, 0.119, and 0.467 as the substrate concentration decreased. Contradictory to our general understanding, acetate production found to have a negligible or even negative effect on the final H<SUB>2</SUB> yield in dark fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Revealing the acetogenic H<SUB>2</SUB>-consuming reaction using FBA in dark fermentation. </LI> <LI> Reutilization of produced H<SUB>2</SUB> by acetogenic H<SUB>2</SUB>-consuming reaction was crucial. </LI> <LI> Acetate production has a negligible or even negative effect on the final H<SUB>2</SUB> yield. </LI> </UL> </P>
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