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권경진 ( Kyoung Jin Kwon ),김도정 ( Do Jung Kim ),김상섭 ( Sang Seop Kim ),최유빈 ( Yu Bin Choi ),김미정 ( Mi Jeong Kim ),최명신 ( Myeong Sin Choi ),최보경 ( Bo Kyung Choi ),김동섭 ( Dong Sup Kim ) 대한화장품학회 2010 대한화장품학회지 Vol.36 No.3
탈모 방지의 목적으로 사용되는 양모제는 현재 우리나라에서 의약외품이며 약사법으로 관리되고 있다. 최근 탈모에 대한 사회적 관심이 증가하면서 양모제에 대한 수요도 증가하고 있는 실정이지만 양모제는 생약추출물, 비타민류, 보존제 등 다양한 종류의 성분이 혼합되어 있어 분석이 쉽지 않다. 본 연구에서는 양모제의 주성분으로 많이 쓰이는 니코틴산아미드, 덱스판테놀, 살리실산, 초산토코페롤, 니코틴산벤질에 대한 동시분석법을 확립하고자 하였다. 시판 중인 양모제를 구입하여 C18 컬럼, 아세토니트릴과 인산염완충액을 용매로 사용하여 HPLC로 분석하였다. 검출은 자외부흡광광도계를 이용하여 220, 270, 300 nm 파장에서 분석하였으며, 12.5 ∼ 800 μg/mL 농도범위에서 검량선을 작성하였다. 회수율은 액상기제의 경우 97.3 ∼ 103.5 % (상대표준편차 0.9 ∼ 2.8 %), 샴푸기제의 경우 101.9 ∼ 115.9 % (상대표준편차 0.7 ∼ 7.7 %)로 양호한 결과를 나타냈다. 시판중인 검체의 함량 시험결과 기준에 적합하였으며, 본 분석법을 이용하여 품질관리 및 추후 양모제 개발에 유용하게 활용함으로써 우수한 품질의 양모제 유통에 크게 기여할 것으로 기대된다. The hair nourisher products are used for prevention of hair loss and classified as quasi-drug in Korea. As concerns about hair loss has been increased, the demand for hair nourisher products has been growing. It is difficult to analyze their main ingredients because they contain various ingredients including natural plant extracts, vitamins, preservatives and exfoliators. The purpose of this study was to develop and validate simultaneous analytical methods of active ingredients in hair nourisher products such as nicotinamide, tocopherol, salicylic acid, dexpanthenol and benzyl nicotinate by HPLC. The active ingredients were separated on a C18 column by using acetonitrile/ phosphate buffer as a mobile phase, and detected at UV 220, 270 and 300 nm. The calibration curve showed linearity in the range of 12.5 ∼ 800 μg/mL and the recoveries were 97.3 ∼ 103.5 % (RSD 0.9 ∼ 2.8 %) in liquid matrix and 101.9 ∼ 115.9 % (RSD 0.7 ∼ 7.7 %) in shampoo matrix. Validated method was applied to hair nourisher products obtained from distribution market. Fortunately, all samples met their criteria. This study might be expected to provide the method for determining active ingredients in hair nourisher products and lead to promote a rapid market entry.
비타민 C와 글루타치온이 수은유도 ROS 생성에 미치는 영향
권경진(Kyoung Jin Kwon),신윤용(Yhun Yhong Sheen) 환경독성보건학회 2008 환경독성보건학회지 Vol.23 No.1
The genotoxicity of mercury compounds have been investigated with a variety of genetic endpoints in prokaryotic and eukaryotic cells. The mercury ions are positively charged and easily form complexes with DNA by binding with negatively charged centers to cause mutagenesis. Further, the mercury ions can react with sulfhydryl (-SH) groups of proteins associated with DNA replication and alter genetic information. Another mechanism by which mercury damages DNA molecule is via its probable involvement of reactive oxygen species (ROS) and induces DNA strand breaks. In order to investigate whether the ROS production was induced by mercury, we performed ROS assay. As the result, the ROS production was significantly increased when it grows dose-dependently and time-dependently. We compared mercury alone-treated group and mercury co-treated with Vitamin C or glutathione group. As the result, the ROS production induced by mercury was decreased by Vitamin C and glutathione. Co-treated with Vitamin C and glutathione group was the most effective to lowering ROS production induced by mercury.
Determination of metabolic enzymes involved in biotransformation of IN-1130, an ALK5 inhibitor
Yeo Woon KIM(김여운),Kyung Nan MIN(민경난),Jung In JEE(지정인),Kyoung Jin KWON(권경진),Seo Youn GO(고서운),Yong Kook KIM(김용국),Il Young CHO(조일영),Youn Jee CHUNG(정연지),Dae-kee KIM(김대희),Yhun Yhong SHEEN(신윤용) 환경독성보건학회 2007 한국독성학회 심포지움 및 학술발표회 Vol.- No.-