http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Determination of metabolic enzymes involved in biotransformation of IN-1130, an ALK5 inhibitor
Yeo Woon KIM(김여운),Kyung Nan MIN(민경난),Jung In JEE(지정인),Kyoung Jin KWON(권경진),Seo Youn GO(고서운),Yong Kook KIM(김용국),Il Young CHO(조일영),Youn Jee CHUNG(정연지),Dae-kee KIM(김대희),Yhun Yhong SHEEN(신윤용) 환경독성보건학회 2007 한국독성학회 심포지움 및 학술발표회 Vol.- No.-
도포형 자기기록 매체의 자성층에서 자성체의 배향거동과 배향상태에 따른 Tape 특성의 변화
김상문(Sang Mun Kim),김태옥(Tae Ok Kim),신학기(Hack Gi Shin),여운성(Woon Seung Yeo) 한국자기학회 1997 韓國磁氣學會誌 Vol.7 No.6
We have observed the orientation behavior of acicular iron oxides in magnetic layer of the particular magnetic tape after magnetic paint was coated on polyester film. As results of the static orientation, orientation of iron oxides come to the maximum at the front and the back of orientation magnets, but it was a litter lower at the back than at the front, and it slowly decreases and come to the constant level after passing through the orientation magnets. In case of edge portion to be futher away from center in the coated film, the orientation of iron oxides in magnetic layer come to worse than that of the center. We think it is owing to the shape and the magnetic magnitude of orientation magnets.<br/> The results of the dynamic orientation are as follows. As the orientation of iron oxides in the particulated magnetic tape is higher, the output properties of tape come to better than ever. And the orientation of iron oxides can be changed by drying condition, as result, the output properties of tape can be also. Therefore we think the considrations of the design of orientation magnets and the control of drying condition are needed to improve output properties of the particulated magnetic tape.
Daisdzein이 Benzo(k)fluoranthene에 의한 CYP1B1 유전자조절 작용에 미치는 영향
서미정,김여운,신윤용,Seo, Mi-Jeong,Kim, Yeo-Woon,Sheen, Yhun-Yhong 한국환경성돌연변이발암원학회 2004 한국환경성돌연변이·발암원학회지 Vol.24 No.4
Cytochrome P4501B1(CYP1B1) is known to be inducible by xenobiotic compounds such as policyclic aromatic hydrocarbon(PAH) and dioxins such as 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). And these induction of CYP1B1 is also regulated by many categories of chemicals. In order to investigate the effects of several chemicals on CYP1B1 gene expression in Hepa-I and MCF-7 cells, 5' flanking DNA of human CYP1B1 was cloned into pGL3 basic vector containing luciferase gene, and then transfected into these cells. After treatment of chemicals, the luciferase activity was measured. CYP1B1 enzyme metabolize PAHs and estradiol. CYP1B1 metabolize estradiol to 4-hydrozyestradiol that is considered as carcinogenic metabolite. Recent industrialized industrialized society, human has been widely been exposed to widespread environmental contaminants such as PAHs(polycyclic aromatic hydrocarbon) that are originated from the imcomplete combustion of hydrocarbons. PAHs are known to be ligands of the AhR(aryl hydrocarbon receptor). Induction of cytochrome P4501B1(CYP1B1) in cell culture is widely used as a biomarker for PAHs. Therefore we have studied the effect of PAHs in the human breast cancer cells MCF-7 to evaluate bioactivity of PAHs. We have used the United State of America EPA selected 13 different PAHs, PAHs mixtures and extracts from environmental samples to evaluate the bioassay system. We examined effects of PAHs on the CYP1B1-luciferase reporter gene and CYP1B1 mRNA level. Benzo(k)fluoranthene and dibenzo(a, h)anthracene showed strong response to CYP1B1 promoter activity stimulation, and also CYP1B1 mRNAs increase in MCF-7 cells in a concentration-dependent manner. RT-PCR analysis indicated that PAHs significantly up-regulate the level of CYP1B1 mRNA. Some flavonoids such as genistein, daidzein, chrysin, naringenin and morin were also investigeted. These flavonoids decreased B(k)F infuced luciferase activity at low concentration. But, these flavonoids exhibited stimulatory effect at high concentration.
Morin이 benzo(k)fluoranthene에 의한 유방암 세포 MCF-7의 CYP1A1 유전자 발현 조절에 미치는 영향
양소연,김여운,신윤용,Yang, So-Yeon,Kim, Yeo-Woon,Sheen, Yhun-Yhong 한국환경성돌연변이발암원학회 2004 한국환경성돌연변이·발암원학회지 Vol.24 No.4
We investigated the effect of dietaty flavonoid, such as CYP1A1 promoter activity, 7-ethoxyresorufin-O-deethylase(EROD) activity and CYP1A1 mRNA expression induced by benzo(k)fluoranthene(B(k)F) in MCF-7 cells. Based on the three criteria of frequency of occurrence in the environment, toxicity and potential exposure to humans, B(k)F is one of the top-listed PAHs. We found that B(k)F significantly up-regulates the level of CYP1A1 promoter activity, EROD and CYP1A1 mRNA. When cells were treated with morin alonem, it was not changed that EROD and CYP1A1 mRNA, compared to that of control. However, morin inhibited the B(k)-induced CYP1A1 prompter activity and mRNA level at high concentration. But morin exhibited stimulatory effects B(k)F-induced CYP1A1 promoter activity and mRNA level at low concentration. Overall, results from these studies demonstrate morin might interfere the action of B(k) with AhR system to stimulate CYP1A1 gene expression. CYP1A1 is known to be inducible by xenobiotic compouds such as polyciclic aromatic hydrocarbons(PAHs) and 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). These chemicals have been identified worldwide and can have a significant impact on the human health and well being of human and wildlife. Given these issues, the detection and quantification of these chemicals in biological, environmental and food samples is important.