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- 저자 : Kuldeepsingh A. Kalariya (검색결과 1 건)
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Identifification of microRNAs from transcriptome data in gurmar (Gymnema sylvestre)
Kuldeepsingh A. Kalariya,Ram Prasnna Meena,Parmeshwar Lal Saran,Ponnuchamy Manivel 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3
MicroRNAs (miRNAs) are single-stranded, non-coding, small (~ 22 nt) RNAs that regulate mRNA targets in plants andanimals. Gymnema sylvestre (Retz.) is an important medicinal plant that lacks genomic as well as transcriptomic information. Here, to identify homologous miRNAs, we screened 6028 unique known plant miRNAs against 272,161 unigenes of G. sylvestre (Retz.) generated by paired-end deep transcriptome sequencing. We utilized 76 aligned unigenes for extracting theprecursor sequences in G. sylvestre (Retz.) and identifi ed 16 potential candidate miRNAs belonging to 12 miRNA families. The mean minimal folding free energy index (MFEI) value of these 16 predicted miRNAs was − 0.966, ranging from − 0.653to − 2.37, while the G + C% content varied between 27.3 and 61.9%. Nucleotide composition analysis revealed that cytosinewas the dominant nucleotide in mature miRNAs (26.3%), while uracil was the next most prevalent nucleotide (25.7%), followedby guanine (24.3%) and adenine (23.7%). In most cases (81%), the fi rst position of the 5′ end was occupied by uracilin the identifi ed mature G. sylvestre (Retz.) miRNAs. Diff erential expression of target unigenes revealed that, as comparedto leaf tissue, a total 16 and 12 target unigenes were diff erentially expressed in fl ower and fruit tissues, respectively. Amongthe diff erentially expressed target unigenes were Auxin Response Factors 17-like, Copia-type polyprotein Phytoene synthase2, as well as several encoding unnamed, predicted, uncharacterized, and/or hypothetical proteins. After detailed predictionof miRNAs and their corresponding target unigenes, we report for the fi rst time a total of 13 miRNA and 213 correspondingtarget unigenes in G. sylvestre (Retz.).
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