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Lee Jung-Sick,Ku Ka-Yeon,Kim Hye-Jin,Park Ji-Seon,Park Jung-Jun,Shin Yun-Kyung,Jeon Mi-Ae 한국발생생물학회 2012 발생과 생식 Vol.16 No.3
This study attempts to propose the possibility of the sex reversal in Tegillarca granosa and Ruditapes philippinarum by confirming the changes in the sex ratio with the shell length (SL) in the same population level. For analysis of sex ratio with SL, 1500 individuals of T. granosa (SL 10.1-45.0 mm) and 712 individuals of R. philippinarum (SL 15.1-70.0 mm) were used. Sex was analyzed histologically. The average sex ratios (F:M) of T. granosa and R. philippinarum were 1:1.22 and 1:0.96, respectively. However, sex ratio was found to differ when the clams were divided into groups according to SL in 5.0 mm intervals. Both species displayed the tendency of increase in the proportion of female with increase in SL. In this study, changes in the sex ratio in accordance with the growth of T. granosa and R. philippinarum are determined to be indirect evidence that signifies their sex reversal.
Han, Yong Duk,Kim, Ka Ram,Park, Yoo Min,Song, Seung Yeon,Yang, Yong Ju,Lee, Kangsun,Ku, Yunhee,Yoon, Hyun C. Elsevier 2017 Materials Science and Engineering C Vol. No.
<P><B>Abstract</B></P> <P>According to recent increases in public healthcare costs associated with diabetes mellitus, the development of new glycemic monitoring techniques based on the biosensing of glycated hemoglobin A1c (HbA<SUB>1c</SUB>), a promising long-term glycemic biomarker, has become a major challenge. In the development of HbA<SUB>1c</SUB> biosensors for point-of-care applications, the selection of an effective biorecognition layer that provides a high reaction yield and specificity toward HbA<SUB>1c</SUB> is regarded as the most significant issue. To address this, we developed a novel HbA<SUB>1c</SUB> biosensing interfacial material by the integration of boronate hydrogel with glass fiber membrane. In the present study, a new boronate-functionalized hydrogel was designed and spatio-selectively photopolymerized on a hydrophilic glass fiber membrane by using N-hydroxyethyl acrylamide, 3-(acrylamido)phenylboronic acid, and bis(<I>N</I>,<I>N</I>′-methylene-bis-acrylamide). Using this approach, the boronic acid group, which specifically recognizes the cis-diol residue of glucose on the HbA<SUB>1c</SUB> molecule, can be three-dimensionally coated on the surface of the glass fiber network with a high density. Because this network structure of boronate hydrogel-grafted fibers enables capillary-driven fluid control, facile HbA<SUB>1c</SUB> biosensing in a lateral flow assay concept could be accomplished. On the proposed HbA<SUB>1c</SUB> biosensing interface, various concentrations of HbA<SUB>1c</SUB> (5–15%) in blood-originated samples were sensitively measured by a colorimetric assay using horseradish peroxidase, a glycoenzyme can generate chromogenic signal after the competitive binding against HbA<SUB>1c</SUB> to the boronic acid residues. Based on the demonstrated advantages of boronate hydrogel-modified membrane including high analytical performance, easy operation, and cost-effectiveness, we expect that the proposed biorecognition interfacial material can be applied not only to point-of-care HbA<SUB>1c</SUB> biosensors, but also to the quantitative analysis of other glycoprotein biomarkers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Boronic acid (BA) hydrogel was synthesized for HbA<SUB>1c</SUB> biosensing. </LI> <LI> BA hydrogel was patterned on the glass fiber membrane via photo-crosslinking. </LI> <LI> HbA<SUB>1c</SUB> and peroxidase were competitively bound to BA hydrogel-modified membrane. </LI> <LI> HbA<SUB>1c</SUB> could be efficiently quantified by peroxidase-based chromogenic reaction. </LI> <LI> HbA<SUB>1c</SUB> in the whole blood sample was determined within a range of 4.6–15.2%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>