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        Identification of <i>Azadinium</i> species and a new azaspiracid from <i>Azadinium poporum</i> in Puget Sound, Washington State, USA

        Kim, Joo-Hwan,Tillmann, Urban,Adams, Nicolaus G.,Krock, Bernd,Stutts, Whitney L.,Deeds, Jonathan R.,Han, Myung-Soo,Trainer, Vera L. Elsevier 2017 HARMFUL ALGAE Vol.68 No.-

        <P><B>Abstract</B></P> <P>The identification of a new suite of toxins, called azaspiracids (AZA), as the cause of human illnesses after the consumption of shellfish from the Irish west coast in 1995, resulted in interest in understanding the global distribution of these toxins and of species of the small dinoflagellate genus <I>Azadinium</I>, known to produce them. Clonal isolates of four species of <I>Azadinium, A. poporum, A. cuneatum, A. obesum</I> and <I>A. dalianense</I> were obtained from incubated sediment samples collected from Puget Sound, Washington State in 2016. These <I>Azadinium</I> species were identified using morphological characteristics confirmed by molecular phylogeny. Whereas AZA could not be detected in any strains of <I>A. obesum, A. cuneatum</I> and <I>A. dalianense</I>, all four strains of <I>A. poporum</I> produced a new azaspiracid toxin, based on LC–MS analysis, named AZA-59. The presence of AZA-59 was confirmed at low levels <I>in situ</I> using a solid phase resin deployed at several stations along the coastlines of Puget Sound. Using a combination of molecular methods for species detection and solid phase resin deployment to target shellfish monitoring of toxin at high-risk sites, the risk of azaspiracid shellfish poisoning can be minimized.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Four <I>Azadinium</I> species were isolated from Puget Sound. </LI> <LI> <I>A. poporum</I> from Puget Sound produce a new azaspiracid, AZA-59. </LI> <LI> AZA-59 was detected at low levels in seawater from several stations in Puget Sound. </LI> </UL> </P>

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