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Coprecipitation of cytochrome C with calcium phosphateon hydroxyapatite ceramic
Yu Sogo,Atsuo Ito,Koshiro Fukasawa,Nao Kondo,Yusuke Ishikawa,Noboru Ichinose,Atsushi Yamazaki 한국물리학회 2005 Current Applied Physics Vol.5 No.5
Cytochrome C (cyt C), which has a molecular weight and an isoelectric point similar to those of broblast growth factor(FGF-2), was coprecipitated with calcium phosphates in the presence or absence of a hydroxyapatite ceramic (HAP) using super-saturated calcium phosphate solutions that can be prepared by mixing infusion uids. The precipitates formed in the solutionconsisted of amorphous calcium phosphate in the absence of a HAP. In the presence of a HAP, precipitates morphologically iden-tical to bone-like apatite formed a calcium phosphate layer on the surface of HAP. The optimum conditions for coprecipitation ofcyt C with calcium phosphate on HAP were a Ca/P molar ratio of 1.5 and a NaHCO3 concentration of 7.90 mM. The release of cytis also expected to be coprecipitated with calcium phosphates onto HAPs using infusion fluids..