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Neš,ović,, Katarina,Janković,, Ana,Kojić,, Vesna,Vukaš,inović,-Sekulić,, Maja,Perić,-Grujić,, Aleksandra,Rhee, Kyong Yop,Miš,ković,-Stankovi Elsevier 2018 Composites. Part B, Engineering Vol.154 No.-
<P><B>Abstract</B></P> <P>This study presents the synthesis of novel silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels by <I>in situ</I> electrochemical reduction of silver ions in the hydrogel matrix and their thorough characterization by UV–visible and Raman spectroscopies, field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and silver release measurements. The influence of chitosan content on the incorporation and stabilization of silver nanoparticles (AgNPs) was investigated, and we found that hydrogels with higher chitosan content contain higher amounts of AgNPs. In addition, the cytotoxicity and antibacterial activity of Ag/PVA/CHI/Gr nanocomposite hydrogels were evaluated. Based on <I>in vitro</I> investigations, the obtained materials exhibit diffusion-controlled release profiles over 28 days, strong antibacterial activity against <I>Staphylococcus aureus</I> and <I>Escherichia coli</I> bacterial strains, and no cytotoxicity toward human and mice fibroblast cell lines.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>In situ</I> electrochemical synthesis of silver nanoparticles in PVA/CHI/Gr hydrogels. </LI> <LI> Effect of chitosan concentration on the amount of embedded silver nanoparticles. </LI> <LI> Effect of chitosan concentration on the silver release kinetics. </LI> <LI> Strong antibacterial activity against <I>S. aureus</I> and <I>E. coli</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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