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      • Effect of Maitake (Grifola frondosa) D-Fraction on the Activation of NK Cells in Cancer Patients

        Noriko Kodama,Kiyoshi Komuta,Hiroaki Nanba 한국식품영양과학회 2003 Journal of medicinal food Vol.6 No.4

        Maitake D-Fraction, extracted from maitake mushroom, has been reported to exert its antitumor effect in tu-mor-bearing mice by enhancing the immune system through activation of macrophages, T cells, and natural killer (NK) cells.In a previous study, the combination of immunotherapy with the maitake D-Fraction and chemotherapy suggested that the D-Fraction may have the potential to decrease the size of lung, liver, and breast tumors in cancer patients. In the present study,we administered maitake D-Fraction to cancer patients without anticancer drugs, and at the same time NK cell activity wasmonitored to investigate whether the activity is closely related with disease progression. The numbers of CD4 1 and CD81cells in the peripheral blood were measured in 10 patients, and NK cell activity was assessed using K-562 cells as target cells.Serum soluble interleukin-2 receptor (sIL-2R) levels in three patients and the expression of tumor markers in four patientswere determined by enzyme-linked immunosorbent assay. The slight changes observed in the CD4 1 and CD81 cell numberswere independent of disease severity or stage as well as serum sIL-2R levels. In contrast, maitake D-Fraction hindered metasta-tic progress, lessened the expression of tumor markers, and increased NK cell activity in all patients examined. Thus maitakeD-Fraction appears to repress cancer progression and primarily exerts its effect through stimulation of NK activity. In addi-tion, we conclude that measurement of NK cell activity may be a useful clinical parameter in monitoring disease progressionduring and following immunotherapy with maitake D-Fraction.

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