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RISS 인기검색어
- 검색키워드
- 저자 : Kelli S. George (검색결과 2 건)
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Seo, Ho Seong,Minasov, George,Seepersaud, Ravin,Doran, Kelly S.,Dubrovska, Ievgeniia,Shuvalova, Ludmilla,Anderson, Wayne F.,Iverson, Tina M.,Sullam, Paul M. American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.50
<▼1><P><B>Background:</B> The serine-rich repeat glycoproteins Srr1 and Srr2 are surface adhesins of <I>Streptococcus agalactiae</I> important for pathogenicity.</P><P><B>Results:</B> Both Srrs bind tandem repeats of the fibrinogen Aα chain, but Srr2 has greater affinity explained by structure-function analysis of the Srrs.</P><P><B>Conclusion:</B> A dock, lock, and latch mechanism describes the Srr-fibrinogen interaction.</P><P><B>Significance:</B> The higher affinity of Srr2 may contribute to the hypervirulence of Srr2-expressing strains.</P></▼1><▼2><P>The serine-rich repeat glycoproteins of Gram-positive bacteria comprise a large family of cell wall proteins. <I>Streptococcus agalactiae</I> (group B <I>streptococcus</I>, GBS) expresses either Srr1 or Srr2 on its surface, depending on the strain. Srr1 has recently been shown to bind fibrinogen, and this interaction contributes to the pathogenesis of GBS meningitis. Although strains expressing Srr2 appear to be hypervirulent, no ligand for this adhesin has been described. We now demonstrate that Srr2 also binds human fibrinogen and that this interaction promotes GBS attachment to endothelial cells. Recombinant Srr1 and Srr2 bound fibrinogen <I>in vitro</I>, with affinities of <I>K<SUB>D</SUB></I> = 2.1 × 10<SUP>−5</SUP> and 3.7 × 10<SUP>−6</SUP><SMALL>M</SMALL>, respectively, as measured by surface plasmon resonance spectroscopy. The binding site for Srr1 and Srr2 was localized to tandem repeats 6–8 of the fibrinogen Aα chain. The structures of both the Srr1 and Srr2 binding regions were determined and, in combination with mutagenesis studies, suggest that both Srr1 and Srr2 interact with a segment of these repeats via a “dock, lock, and latch” mechanism. Moreover, properties of the latch region may account for the increased affinity between Srr2 and fibrinogen. Together, these studies identify how greater affinity of Srr2 for fibrinogen may contribute to the increased virulence associated with Srr2-expressing strains.</P></▼2>
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Sarah A. Johnson,Negin Navaei,Shirin Pourafshar,Salvador J. Jaime,Neda S. Akhavan,Stacey Alvarez-Alvarado,Gabriela V. Proano,Nicole S. Litwin,Elizabeth A. Clark,Elizabeth M. Foley,Kelli S. George,Marc 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.12
Greater than one-third of adults in the United States have metabolic syndrome (MetS), a cluster of risk factors highly associated with the development of cardiovascular diseases. Premature vascular dysfunction in MetS may lead to accelerated age-related atherogenesis and arterial stiffening, thereby increasing cardiovascular risk. Montmorency tart cherries (Prunus cerasus L.) are rich in bioactive compounds, such as anthocyanins, known to exert cardiovascular protective effects. Previous research suggests that tart cherry juice consumption may improve cardiovascular health. The objective of this study was to evaluate the effects of daily consumption of tart cherry juice on hemodynamics, arterial stiffness, and blood biomarkers of cardiovascular and metabolic health in men and women with MetS. In a randomized, single-blind, placebo-controlled, parallel-arm pilot clinical trial, 19 men and women 20 to 60 years of age with MetS consumed 240 mL of tart cherry juice (Tart Cherry; n = 5 males, 4 females) or an isocaloric placebo-control drink (Control; n = 5 males, 5 females) twice daily for 12 weeks. Arterial stiffness (pulse wave velocity), brachial and aortic blood pressures, wave reflection (augmentation index), and blood biomarkers of cardiovascular and metabolic health were assessed at baseline and 6 and 12 weeks. Oxidized low-density lipoprotein and soluble vascular cell adhesion molecule-1 were significantly lower (P = .047 and P = .036, respectively) in Tart Cherry than Control at 12 weeks, but were not significantly lower than baseline values. There was a trend for total cholesterol to be lower (P = .08) in Tart Cherry than Control at 12 weeks. No significant changes were observed in hemodynamics, arterial stiffness, or other blood biomarkers assessed. These results suggest that daily tart cherry consumption may attenuate processes involved in accelerated atherogenesis without affecting hemodynamics or arterial stiffness parameters in this population. The pilot nature of this study warrants interpreting these findings with caution, and future clinical trials with a larger sample size are needed to confirm these findings.
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