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RISS 인기검색어
- 검색키워드
- 저자 : Keely, Patricia J. (검색결과 2 건)
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Moon, Ju-Yeon,McNamara, Keely May,Lee, Jung-Jin,Chung, Bong Chul,Sasano, Hironobu,Choi, Man Ho Elsevier 2018 The Journal of steroid biochemistry and molecular Vol.178 No.-
<P><B>Abstract</B></P> <P>Sex steroids in clinical endocrinology have been mainly investigated with peripheral blood and urine samples, while there is limited information regarding the local levels within tissues. To improve analytical properties of sex steroids from trace amounts of tissue samples, two-phase extractive ethoxycarbonlyation and subsequent pentafluoropropionyl derivatization coupled to gas chromatography–tandem mass spectrometry (GC–MS/MS) was developed. The optimized analytical conditions led to excellent chromatographic separation of 15 estrogens, 6 androgens, and 2 progestins. The quantitative results were calculated based on <I>in-house</I> control samples as the steroid-free tissues, and the precision and accuracy were 4.2%–26.8% and 90.8%–116.4%, respectively. The on-column limit of quantification was from 180 fg to 0.5 pg for androgens and estrogens, and 1.25 pg for progestins, which were found to be linear (<I>r</I> <SUP>2 </SUP>> 0.990). The validated method was then applied to quantify 7 sex steroids from three 100-μm-thick frozen breast tissue slices from postmenopausal patients with breast cancer. This is the first report on the improved GC–MS/MS method for the detection of androgens and pregnenolone from breast cancer tissues, and it can be a useful technique to measure the local levels of sex steroids, thus, enhancing our understanding of the pathophysiological significances of steroidogenesis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sex steroids were measured from trace amounts of tissue samples. </LI> <LI> Extractable alkyloxycarbonylation improved chromatographic properties. </LI> <LI> Pentafluroropropionylation produces characteristic ions in mass spectrometry. </LI> <LI> GC–MS/MS resulted in a sensitive detection of sex steroids from frozen breast tissue slices. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Conklin, Matthew W.,Keely, Patricia J. Landes Bioscience 2012 Cell adhesion & migration Vol.6 No.3
<P>Survival and recurrence rates in breast cancer are variable for common diagnoses, and therefore the biological underpinnings of the disease that determine those outcomes are yet to be fully understood. As a result, translational medicine is one of the fastest growing arenas of study in tumor biology. With advancements in genetic and imaging techniques, archived biopsies can be examined for purposes other than diagnosis. There is a great deal of evidence that points to the stroma as the major regulator of tumor progression following the initial stages of tumor formation, and the stroma may also contribute to risk factors determining tumor formation. Therefore, aspects of stromal biology are well-suited to be a focus for studies of patient outcome, where statistical differences in survival among patients provide evidence as to whether that stromal component is a signpost for tumor progression. In this review we summarize the latest research done where breast cancer patient survival was correlated with aspects of stromal biology, which have been put into four categories: reorganization of the extracellular matrix (ECM) to promote invasion, changes in the expression of stromal cell types, changes in stromal gene expression, and changes in cell biology signaling cascades to and from the stroma.</P>
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