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      • KCI등재

        DNA Microarray Analysis on Saccharomyces cerevisiae under High Carbon Dioxide Concentration in Fermentation Process

        Hiroshi Shimizu,Keisuke Nagahisa,Toshiharu Nakajima,Katsunori Yoshikawa,Takashi Hirasawa,Yoshio Katakura,Chikara Furusawa,Suteaki Shioya 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.5

        The effect of carbon dioxide on yeast growth was investigated during the cultivation of pH 5.0 and pH 6.8, by replacing the nitrogen part with carbon dioxide under aerobic conditions. The values of the specific growth rate under pH 5.0 and pH 6.8 conditions became 64.0% and 46.9%, respectively, compared to those before the change in gas composition. This suggests that the effect of carbon dioxide was greater pronounced in pH 6.8 than in pH 5.0. The genome-wide transcriptional response to elevated carbon dioxide was examined using a DNA microarray. As for upregulated genes, it was noteworthy that 3 genes were induced upon entry into a stationary phase and 6 genes were involved in stress response. Of 53 downregulated genes, 22 genes were involved in the ribosomal biogenesis and assembly and 5 genes were involved in the lipid metabolism. These facts suggest that carbon dioxide could bring the cell conditions partially to a stationary phase. The ALD6 gene encoding for cytosolic acetaldehyde dehydrogenase was downregulated, which would lead to a lack of cell components for the growth. The downregulation of ALD6 was greater in pH 6.8 than in pH 5.0, consistent with physiological response. This suggests that it might be the most effective factor for growth inhibition.

      • SCIESCOPUSKCI등재

        DNA Microarrav Analysis on Saccharomyces cerevisiae under High Carbon Dioxide Concentration in Fermentation Process

        Nagahisa, Keisuke,Nakajima, Toshiharu,Yoshikawa, Katsunori,Hirasawa, Takashi,Katakura, Yoshio,Furusawa, Chikara,Shioya, Suteaki,Shimizu, Hiroshi The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.5

        The effect of carbon dioxide on yeast growth was investigated during the cultivation of pH 5.0 and pH 6.8. by replacing the nitrogen part with carbon dioxide under aerobic conditions. The values of the specific growth rate under pH 5.0 and pH 6.8 conditions became 64.0% and 46.9%, respectively, compared to those before the change in gas composition. This suggests that the effect of carton dioxide was greater pronounced in pH 6.8 than in pH 5.0. The genome-wide transcriptional response to elevated carbon dioxide was examined using a DNA microarray. As for upregulated genes, it was noteworthy that 3 genes were induced upon entry into a stationary phase and 6 genes were involved in stress response. Of 53 downregulated genes, 22 genes were involved in the ribosomal biogenesis and assembly and 5 genes were involved in the lipid metabolism. These facts suggest that carbon dioxide could bring the cell conditions partially to a stationary phase. The ALD6 gene encoding for cytosolic acetaldehyde dehydrogenase was downregulated, which would lead to a lack of cell components for the growth. The downregulation of ALD6 was greater in pH 6.8 than in pH 5.0. consistent with physiological response. This suggests that it might be the most effective factor for growth inhibition.

      • SCOPUSKCI등재

        An extension plan of Yakushima Biosphere Reserve as a case study of consensus building of islanders

        Matsuda, Hiroyuki,Yumoto, Takakazu,Okano, Takahiro,Tetsuka, Kenshi,Fujimaki, Aomi,Shioya, Katsunori The Ecological Society of Korea 2015 Journal of Ecology and Environment Vol.38 No.2

        A participatory approach of local stakeholders is important to create a balance between nature protection and the sustainable use of natural resources in a nature reserve and its vicinity area. The zoning and management policy of biosphere reserves (BRs) is a good example of this idea, especially for island reserves. Yakushima was inscribed as a World Natural Heritage (WH) in 1993. Almost all of its residents did not remember that Yakushima was a BR. In addition, Yakushima has some problems in nature reserve management. Overabundance of the deer population is a critical threat to both agriculture and natural vegetation in the world heritage site. There are too many tourists visiting the WH site and Nagata-hama beach. The beach is a Ramsar site and one of the most important spawning grounds of sea turtles in the north Pacific. Now, residents and the municipality have decided to reactivate the Yakushima BR. Although the Ministry of the Environment of Japan has an initiative to manage the WH and the Ramsar site, the BR is managed by the municipality and local stakeholders. Local activities of both nature conservation and the sustainable use of natural resources are encouraged in the BR site. The islanders willingly included all areas of the island into the BR site. Scientists who have participated in the Society of Yakushimaology have played a significant role to build trust between local stakeholders and to seek feasible policies on the nature reserves. The concept of the "Yakushima Environmental Culture Village" is a good model for the extension plan of the Yakushima and Kuchinoerabujima BRs.

      • KCI등재

        An extension plan of Yakushima Biosphere Reserve as a case study of consensus building of islanders

        Hiroyuki Matsuda,Takakazu Yumoto,Takahiro Okano,Kenshi Tetsuka,Aomi Fujimaki,Katsunori Shioya 한국생태학회 2015 Journal of Ecology and Environment Vol.38 No.2

        A participatory approach of local stakeholders is important to create a balance between nature protection and the sus¬tainable use of natural resources in a nature reserve and its vicinity area. The zoning and management policy of biosphere reserves (BRs) is a good example of this idea, especially for island reserves. Yakushima was inscribed as a World Natural Heritage (WH) in 1993. Almost all of its residents did not remember that Yakushima was a BR. In addition, Yakushima has some problems in nature reserve management. Overabundance of the deer population is a critical threat to both agricul¬ture and natural vegetation in the world heritage site. There are too many tourists visiting the WH site and Nagata-hama beach. The beach is a Ramsar site and one of the most important spawning grounds of sea turtles in the north Pacific. Now, residents and the municipality have decided to reactivate the Yakushima BR. Although the Ministry of the Environ-ment of Japan has an initiative to manage the WH and the Ramsar site, the BR is managed by the municipality and local stakeholders. Local activities of both nature conservation and the sustainable use of natural resources are encouraged in the BR site. The islanders willingly included all areas of the island into the BR site. Scientists who have participated in the Society of Yakushimaology have played a significant role to build trust between local stakeholders and to seek feasible policies on the nature reserves. The concept of the “Yakushima Environmental Culture Village” is a good model for the extension plan of the Yakushima and Kuchinoerabujima BRs.

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