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- 저자 : KarlFrancis (검색결과 1 건)
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Characterization of the KG1a Cell Line for Use in a Cell Migration Based Screening Assay
KarlFrancis,이균민,BernhardO.Palsson 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.3
for potentially therapeutic compounds. Further screening of these lead compounds is typicaly done with secondary assays which may utilize living, functioning cels as screening tools. A prob-lem (or benefit) with these cell-based assays is that living cells are very sensitive to their environ-ment. We have been interested in the process of stem cel migration and how it relates to the cel-lular therapy of bone marrow transplantation. In this study we describe a secondary, cel-based as-say for screening the effects of various in-vitro conditions on Imature Hematopoietic Cell (IHC) or the effect of a cultures growth phase, that need to be accounted for in a screning protocol. Fi-nally, we show that exponentially growing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly sugest that KG1a cels secrete a chemokinetic factor during the exponential growth phase of a culture.
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