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      • KCI등재

        Studies on growth kinetics of predominantly Pseudomonas sp. in internal loop airlift bioreactor using phenol and m-cresol

        Pichiah Saravanan,Kannan Pakshirajan,Prabirkumar Saha 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.7

        Growth profile of predominantly Pseudomonas species was studied using wastewater containing phenol and m-cresol, as single and multi component systems in an internal loop airlift bioreactor (ILALR). The species utilized for the study was isolated from a wastewater treatment plant. The reactor was operated at both lower and higher hydraulic retention time (HRT_s), 4.1 h and 8.3 h, respectively. The inlet phenol and concentration was varied between 100 and 800 mg/L with 800 mg/L as shock loading concentration for an HRT of 8.3 h. For 4.1 h HRT, the concentration was varied 100 and 500 mg/L using 500 mg/L as a shock loading concentration. The study showed complete degradation of both phenol and m-cresol, when present individually at an HRT of 8.3 h with an enriched biomass output. The specific growth rate of the culture at various phenol and m-cresol concentrations was fitted to a Monod model. The biokinetics value showed good potential of Pseudomonas species employing the internal loop air lift bioreactor in utilizing high strength phenolics containing wastewater. Culture growth profile with both phenol and m-cresol as mixtures also showed decreased lag times with complete utilization of the phenolics.

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        Removal of Cu(II) by biosorption onto coconut shell in fixed-bed column systems

        Mike A. Acheampong,Kannan Pakshirajan,Ajit P. Annachhatre,Piet N.L. Lens 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.3

        Biosorption of Cu(II) onto coconut shell, an agricultural biomaterial, was studied in a fixed-bed column. The Cu(II) biosorption column had the best performance at 10 mg Lˉ1 inlet Cu(II) concentration, 10 mL minˉ1 flow rate and 20 cm bed depth. The equilibrium uptake of Cu(II) amounted to 7.25 mg gˉ1. The simulation of the breakthrough curve was successful with the BDST and Yoon–Nelson models, but the entire breakthrough curve was best predicted by the Clark model. The design of a fixed bed column for Cu(II)removal from wastewater by biosorption onto coconut shell can be done based on these models.

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        Cadmium removal by Anabaena doliolum Ind1 isolated from a coal mining area in Meghalaya, India

        Smita Goswami,Mayashree B. Syiem,Kannan Pakshirajan 대한환경공학회 2015 Environmental Engineering Research Vol.20 No.1

        The cyanobacterium Anabaena doliolum Ind1 isolated from a coal mining site was tested for removal of cadmium at optimum pH 7.0 and temperature 25°C. The organism recorded high percentage of metal removal (92-69%) within seven days of exposure to 0.5-2.0 ppm cadmium. Biosorption onto the cell surface was the primary mode of metal removal. Fourier transform infrared spectroscopy (FTIR) established hydroxyl, amides, carboxyl, sulphate and carbonyl groups to be the major functional groups on the cell surface involved in cadmium binding. Cellular ultrastructure and a range of vital physiological processes (i.e., photosynthetic pigments, respiration, photosynthesis, heterocyst frequency and nitrogenase activity) remained unaffected upon 0.5 ppm treatment; higher concentrations of cadmium exerted visible adverse effects. Amongst the five photosynthetic pigments tested, phycocyanin was the most targeted pigment (inhibition was 15-89%). Both respiration and photosynthetic activities were inhibited by cadmium with more severe effect seen on respiration. 2.0 ppm cadmium exposure also had drastic negative effect on nitrogenase activity (87% decreased).

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        Kinetics of growth on dual substrates, production of novel glutaminase-free L-asparaginase and substrates utilization by Pectobacterium carotovorum MTCC 1428 in a batch bioreactor

        Kumar Sanjay,Ashish Prabhu Anand,Venkata Dasu Veeranki,Pakshirajan Kannan 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.1

        Bacterial L-asparaginase has been widely used as a potential therapeutic agent in the treatment of various lymphoblastic leukemia diseases. We studied product and dual substrates utilization kinetics by P. carotovorum MTCC 1428 in batch bioreactor. The kinetic study revealed that the maximum growth of P. carotovorum MTCC 1428 was achieved at 2 g l−1 and 5 g l−1 of glucose and L-asparagine, respectively. Different substrate inhibition models were fitted to the growth kinetic data and the additive form of double Luong model was found to best explain the growth kinetics of P. carotovorum MTCC 1428. The kinetic parameters of growth studies showed that the predicted maximum inhibition concentration of glucose (Smg) and L-asparagine (Sma) was close to the experimentally observed value 15.0 and 10 g l−1, respectively. Modified form of the Luedeking-Piret model was used to describe the kinetics of L-asparaginase production, and the system seems to be mixed growth associated. Kinetic models of dual substrate growth, L-asparaginase production and substrate(s) utilization by P. carotovorum MTCC 1428 well fitted with experimental data with regression coefficients (R2) value of 0.97, 0.96 and 0.93, respectively.

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