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The application of modified isochronal well test in a low-permeability condensate gas field
Zhaojing Zhou,Xiuwei Wang,Xue Miao,Kangning Qian,Lijian Li,Peng Xu,Xiuqin Lu 한국자원공학회 2019 Geosystem engineering Vol.22 No.6
The modified isochronal well test is a deliverability test method suitable for gas wells in low-permeability gas reservoirs. Generally, the absolute open flow of gas wells is determined by modified isochronal well test, thereby establishing a reasonable working system for gas wells. It is also possible to obtain gas layer physical parameters, gas reservoir engineering parameters, heterogeneity, boundary characteristics, etc. In this paper, the actual test data of a condensate gas field are applied. By modified isochronal well test analysis, not only the deliverability equation of the gas well is obtained, but also the absolute open flow is determined. At the same time, the formation parameters such as reservoir permeability and skin factor are obtained and the acidification effect is evaluated.
Yu, Changsong,Jia, Gang,Jiang, Yi,Deng, Qiuhong,Chen, Zhengli,Xu, Zhiwen,Chen, Xiaolin,Wang, Kangning Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5
The glucagon-like peptide 2 (GLP-2) that is expressed in intestine epithelial cells of mammals, is important for intestinal barrier function and regulation of tight junction (TJ) proteins. However, there is little known about the intracellular mechanisms of GLP-2 in the regulation of TJ proteins in piglets' intestinal epithelial cells. The purpose of this study is to test the hypothesis that GLP-2 regulates the expressions of TJ proteins in the mitogen-activated protein kinase (MAPK) signaling pathway in piglets' intestinal epithelial cells. The jejunal tissues were cultured in a Dulbecco's modified Eagle's medium/high glucose medium containing supplemental 0 to 100 nmol/L GLP-2. At 72 h after the treatment with the appropriate concentrations of GLP-2, the mRNA and protein expressions of zonula occludens-1 (ZO-1), occludin and claudin-1 were increased (p<0.05). U0126, an MAPK kinase inhibitor, prevented the mRNA and protein expressions of ZO-1, occludin, claudin-1 increase induced by GLP-2 (p<0.05). In conclusion, these results indicated that GLP-2 could improve the expression of TJ proteins in weaned pigs' jejunal epithelium, and the underlying mechanism may due to the MAPK signaling pathway.