http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kailash Choudhary,M. Singh,M. S. Rathore,N. S. Shekhawat 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.3
An efficient in vitro regeneration protocol for moth bean [Vigna aconitifolia (Jacq.) Marechal] via somatic embryogenesis has been developed. Embryogenic callus cultures were established from the cotyledonary node as explant on semi-solid Murashige and Skoog (MS) medium supplemented with 0.75 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.5 mg l-1 6-benzylaminopurine (BA) and with various additives (50 mg l-1 ascorbic acid and 25 mg l-1 each of adenine sulphate, citric acid and L-arginine). Numerous somatic embryos differentiated on MS basal nutrient medium supplemented with 0.25 mg l-1 2,4-D and 0.5 mg l-1 of kinetin (Kin). Sustained cell division resulted in the formation of cell aggregates, which progressed to the globular- and heartshaped somatic embryos and then, if they differentiated properly, to the torpedo shape and cotyledonary stages. The transfer of embryos onto fresh MS basal medium containing 0.2 mg l-1 BA and 2.0 mg l-1 gibberellic acid enabled the embryos to achieve complete maturation and germination. More than 80% of somatic embryos were converted into true-to-type fertile plants. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil. An efficient in vitro regeneration protocol for moth bean [Vigna aconitifolia (Jacq.) Marechal] via somatic embryogenesis has been developed. Embryogenic callus cultures were established from the cotyledonary node as explant on semi-solid Murashige and Skoog (MS) medium supplemented with 0.75 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.5 mg l-1 6-benzylaminopurine (BA) and with various additives (50 mg l-1 ascorbic acid and 25 mg l-1 each of adenine sulphate, citric acid and L-arginine). Numerous somatic embryos differentiated on MS basal nutrient medium supplemented with 0.25 mg l-1 2,4-D and 0.5 mg l-1 of kinetin (Kin). Sustained cell division resulted in the formation of cell aggregates, which progressed to the globular- and heartshaped somatic embryos and then, if they differentiated properly, to the torpedo shape and cotyledonary stages. The transfer of embryos onto fresh MS basal medium containing 0.2 mg l-1 BA and 2.0 mg l-1 gibberellic acid enabled the embryos to achieve complete maturation and germination. More than 80% of somatic embryos were converted into true-to-type fertile plants. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil.