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- 저자 : Jong Won Joo(주종원) (검색결과 4 건)
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PCR을 이용한 육류 내 Campylobacter sp. 및 Campylobacter jejuni의 분리 검출
주종원(Jong-Won Joo),홍경표(Kyung-Pyo Hong),김용휘(Yong-Hui Kim),조상범(Sang-Buem Cho) 동아시아식생활학회 2008 동아시아식생활학회지 Vol.18 No.5
The principal objective of this study was to develop the optimum oligonucleotide primers for the simple detection of Campylobacter in food samples. In order to achieve this goal, a variety of oligonucleotide primers were designed via the modification of 16S rDNA, ceuE and mapA sequences of Campylobacter. Through the subsequent analysis of the specificity and sensitivity of primers, two types of oligonucleotide primers, CB4 and CJ1, were selected for Campylobacter genus-specific and C. jejuni species-specific primers, respectively. The detection limit was found to be 10?~10¹ cells per reaction with the prepared cell suspension, however, the sensitivity in the meat samples was less, at 10¹~10². We suggested that PCR inhibitors such as hemoglobin or immunoglobulin in pork or beef influenced.
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PCR을 이용한 육류 내 Salmonella sp. 및 Salmonella Typhimurium 분리 검출
주종원(Jong-Won Joo),홍경표(Kyung-Pyo Hong),김용휘(Yong-Hui Kim),조상범(Sang-Buem Cho) 동아시아식생활학회 2009 동아시아식생활학회지 Vol.19 No.2
The specificity and sensitivity of oligonucleotide primers were examined for the rapid detection of Salmonella in meat samples. The oligonucleotide primers used in this study were designed with the modification of mdh and invA sequence in the chromosome of Salmonella Typhimurium. Through the subsequent analysis of the specificity and sensitivity of the primers, two types of oligonucleotide primers, SLM1 and SLT4 were selected for the detection of Salmonella genus specific and S. Typhimurium species specific, respectively. The lowest detection limit of each primer was represented as 1 cell per reaction when reacted with a prepared DNA solution. The detection efficiency of the two primers was analysed with beef and pork samples intentionally contaminated with a mixture of Salmonella culture, and three preparation methods -, namely direct reaction after extraction, enrichment after extraction, and DNA extraction after enrichment for PCR reaction, - were also compared. No differences were found in the results according to meat sources and preparation methods.
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Effects of High Protein Diet on Meat Productivity and Quality in Multiparous Hanwoo Cull Cows
Do Hyeong Lee(이도형),Woo Jung Yoon(윤우정),Nag-Jin Choi(최낙진),Kyeong Seon Ryu(류경선),Young Kyoon Oh(오영균),Sun Sik Jang(장선식),Chang Weon Choi(최창원),Jong Won Joo(주종원),Sang Buem Cho(조상범),Eun Joong Kim(김은중) 한국생명과학회 2011 생명과학회지 Vol.21 No.9
일반적으로 다산 한우암소는 미경산 혹은 어린 한우암소에 비하여 낮은 육질을 나타내는 것으로 알려져 있으며, 현재까지 다산 한우 암소 비육을 위한 사료급여방법에 대한 연구는 매우 미비하다. 본 연구는 두 개의 서로 다른 사료로 구성된 사료급여방법이 다산 한우 암소의 육생산과 육질에 미치는 영향을 조사하였다. 대조구로는 일반 비육우사료 급여를 사용하였다. 처리구의 사료는 두 개의 서로 다른 단백질 수준으로 구성되어 있으며 14.28%의 단백질 사료는 비육초기에 급여하였으며, 12.70%의 단백질 수준은 출하 전까지 급여하였다. 대조구는 일반비육우사료(12.39% CP)를 사용하였다. 사양실험은 3개의 농장에서 수행되었다. 농장A에서는 29두의 다산 한우암소를 처리구 그리고 3두를 대조구로 공시하였다. 농장B와 농장C에서는 처리구와 대조구에 각각 8두와 3두 그리고 11두와 4두를 공시하였다. 실험사료들은 총 211일 동안 급여하였고, 처리구의 비육전기사료는 4개월간 급여하였고, 나머지는 비육후기 사료를 급여하였다. 평균일당증체량, 등 지방두께, 등심면적, 도체중, 육량지수, 근내지방, 육색 및 조직감등을 분석하였다. 통계처리는 렌덤효과모형[8]을 사용하였다. 처리의 정적효과는 평균일당 증체량, 등지방두께, 등심면적, 도체중 및 근내지방에서 나타났으며, 육량지수, 육색 및 조직감은 처리구의 부적효과가 나타났다. 모든 분석항목에서 통계적 유의성이 발견되지 않았는데 이것은 농장별 실험결과의 분산이 매우 켰기 때문이다. 그러나 통합적 효과에 있어 평균일당증체량, 등지방두께, 등심면적 및 도체중에 대한 90% 신뢰구간은 정적효과 방향으로 나타났으며, 나머지 분석항목의 90% 신뢰구간은 부적효과의 방향으로 나타났다. 결과적으로 다산 한우암소비육에 있어 비육전기기간의 고단백질 사료 급여는 육생산성 향상 및 육질 개선의 효과를 나타낼 수 있다. Multiparous Hanwoo cull cows have been generally regarded to have poor meat quality compared to young and primiparous Hanwoo in Korea, and there have been few studies attempting to understand various feeding programs for the multiparous Hanwoo cull cow. In this study, the effects of a feeding program consisting of two different diets for multiparous Hanwoo cull cows on meat production and quality were tested in comparison to a commercially used diet. Diets for treatment consisted of two levels of crude protein contents, 14.28% and 12.70% for early fattening and finishing, respectively. For a control, commercially used fattening feed (12.39% crude protein) was used. Feeding trials were performed at three different farms. In farm A, 29 herds of multiparous Hanwoo cull cows were used for the treatment group and 3 herds for the control. In farms B and C, the number of animal herds for treatment and control were 8 vs. 3 and 11 vs. 4 herds, respectively. Experiment diets were fed for an average of 211 days and in treatment, early fattening diet was fed for 4 months and then finishing feed was fed until slaughter. Average daily gains, thickness of back fat, area of Longissimus dorsi, carcass weight, index of carcass weight, intramuscular fat, meat color and texture were analyzed after slaughter. Random effect model [8] was employed in effect analysis. Positive effects of treatment were found in terms of average daily gain, back fat thickness, Longissimus dorsi area, carcass weight, and intramuscular fat. Carcass yield index, meat color and texture showed a negative effect. In this study, significant results were not found in all factors analyzed because the variance between experiment farms was large, however the 90% confidence interval of summary effects of ADG, back fat thickness, Longissimus dorsi area and carcass weight were significant and that of carcass yield index, intramuscular fat, meat color and texture were less so. Conclusively, a high protein diet fed early during the fattening period in multiparous Hanwoo cull cows could have positive effects on meat production.
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