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- 저자 : Jingwan Yan (검색결과 2 건)
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Yan Jingwan,Lin Zhimin,Hu Changquan,Wang Feng 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.6
A new phosphatidylinositol transporter gene was isolated from the T-DNA insertion mutant libraries of rice. The RT-PCR and GUS staining showed that it belonged to embryo specific expression gene. The phonotype of growth and development revealed that the growth was slower and leaf yellowing than that of wild type before 5-leaf stage in rice seedlings, and it was completely restored until to 5-leaf stage. Further, the transcriptome sequencing results showed that the gene affected the nitrogen metabolism signal pathway between mutant and wild type. In addition, our study revealed an important phosphati- dylinositol transporter protein which can control the growth development and promote nitrate transporter and absorption in rice seedling stage.
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Characterization of a strong green tissue-specific motif in rice photosystem I gene promoter Ppsak
Zhimin Lin,Jingwan Yan,Huanxin Yan,Feng Wang 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.2
Characterization of green tissue-specific promoters helps facilitate genetic improvement in crops. Here, we isolated a novel green tissue-specific expression gene Psak encoding photosystem I protein in rice through RTPCR analysis. The 5′ flanking region from −635 to +60 (transcription start site of Psak as +1) covering parts of Psak gene and its promoter Ppsak was identified as be critical for green tissue-specific expression (including leaves and stems) in rice. Further promoter deletion analyses demonstrated that the promoter region from −559 to −232 was necessary and sufficient for green tissue-specific expression of Psak gene. Histochemical assays showed that the GUS expression of this 267-bp region was highly presented in leaves and stems, but reduced or absent in other tissues examined. The GUS expression level of this core promoter region could be 14.6- to 21.6-fold higher than that of the full-length region of Ppsak promoter in leaves and stems. These results combined with cis-acting elements prediction in Ppsak promoter suggested that the TCT motif located in this promoter played a key role in strong green tissue-specific expression.
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