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Jianrui Sun,Jinglan Li,Linlin Yao,Yingying Zheng,Jiang-Feng Yuan,Da-Hong Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.3
Natamycin is widely used in food, medical and health, agriculture, and animal husbandry. In this study, Streptomyces natalensis HW-2 was used as the research object, and a mutant DES-26 with stable genetic characters was selected by UV-ARTP-DES compound mutation. The natamycin yield was 1.64 g/L, 86.36% higher than original strain. Differential expression genes were analyzed by transcriptomics, and results showed that 295 and 860 genes were significantly differentially expressed at fermentation for 48 h and 72 h. GO and KEGG analysis showed that compound mutagenesis had a significant impact on glycolysis, pentose phosphate, TCA cycle, fatty acid metabolism pathways, and several key enzyme genes in the pathways were up-regulated, and genes related to natamycin biosynthesis (pimB-pimI) and transcriptional regulator (pimR) were also up-regulated. qRT-PCR results confirmed that expression levels of these genes were consistent with transcriptional changes of RNA-Seq.
Shuying Dong,Xuanxuan Yan,Wenli Li,Yafei Liu,Xiaoxu Han,Xiaodan Liu,Jinglan Feng,Chongfei Yu,Chunyan Zhang,Jianhui Sun 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-
In order to obtain a robust, durable and efficient heterogeneous catalyst, macroscopic monolithic Zndopeda-Fe2O3/graphene aerogel (GA) hybrid architecture with integrated morphology and hierarchicallyporous structure were controllably synthesized via a facile in-situ hydrothermal method and then used aspersulfate (PS) activator for sulfamonomethoxine (SMM) wastewater purification. Several key reactionparameters including the initial SMM concentration, reaction temperature, coexisting inorganic anionsand SMM in real natural water samples had different influence on the SMM removal efficiency. The catalyticefficiency of Zn-doped a-Fe2O3/GA with the molar ratio of Fe/Zn = 2:1.5 was about 66%, 62%, 66%and 11%33% higher than that of GA, a-Fe2O3/GA, Zn/GA and other Fe/Zn molar ratio. The improvedactivity of Fe/Zn = 2:1.5 benefits from the synergistic effects of the sp2 hybridized carbon and porousframework, as well as the surface oxygenic functional groups, which accelerate the pollutant/oxidant dispersionand electron transfer. Electron paramagnetic resonance results indicate that OH, 1O2 and SO4radicals account for the catalytic degradation of SMM and the activation of PS in present system is differentfrom conventional homogeneous systems, and speculate mechanism was proposed based on theobtained data.