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RISS 인기검색어
- 검색키워드
- 저자 : Jianya Su (검색결과 4 건)
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Xi Wang,Linlin Lou,Jianya Su 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
The field population of Spodoptera litura from Huizhou, Guangdong Province, China was evaluated for resistance to 21 insecticides, including conventional and new chemistry insecticides. Extreme levels of resistance were observed to metaflumizone and emamectin benzoate with resistance factors of 234.1 and 183.3, respectively. Resistance to abamectin was also high (perhaps extremely high) and over 71.9-fold. The Huizhou population of S. litura possessed high resistance to deltamethrin (96.5-fold) and moderate resistance to beta cyfluthrin and lambda cyhalothrin but remained susceptible to bifenthrin. Moderate resistance to chlorantraniliprole (22.3-fold), endosulfan (22.2-fold), tebufenozide (10.7-fold) and thiodicarb (14.3-fold), and low-level resistance to fipronil, indoxacarb and spinosad were also reported in this population. This field population remained susceptible to acetamiprid, chlorfenapyr, chlorfluazuron, hexaflumuron, chlorpyrifos, pyridalyl and spinetoram. The stabilities of resistance to metaflumizone, emamectin benzoate, deltamethrin, chlorantraniliprole and endosulfan were evaluated, the resistance level decreased when the insecticide stress was removed, suggesting stop of the application of insecticides with high level resistance could be implemented into the resistance management. Because S. litura from Huizhou developed resistance to multiple insecticides, integration of different control practices, especially the rotation of insecticides with biocontrol agents, should be performed in the management of this pest. The results suggested the suspension of the application of insecticide to which S. litura had developed high level of resistance in order to mitigate the resistance status, and the use of the insecticides to which this pest remained sensitive, including spinetoram, pyridalyl, indoxacarb, hexaflumuron, chlorfluazuron, chlorfenapyr and bifenthrin, could be incorporated into the alternating application for resistance management.
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Shiyu Wang,Bo Hu,Qi Wei,Jianya Su 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2
Hexamerins are hemolymph-proteins, which are mainly considered as storage proteins for non-feeding stages, and also undertake other roles during insect development and growth, however the characterization of hexamerin proteins in Spodoptera exigua is less understood. In this study five new hexamerin genes were identified and a total seven hexamerin genes were reported in S. exigua. These hexamerins contain the typical domains of hemocyanin at the N-terminal, C-terminal and in the middle of their protein sequences. These genes are mainly expressed in fat body, and the signal peptide sequences at their N-terminal of protein sequences can drive the expressed protein to excrete into hemolymph after synthesis. The phylogenetic analysis and amine acid composition revealed S. exigua express five different types of hexamerins: 1) Storage protein rich in methionine residue (MRSP), 2) Storage protein moderately rich in methionine (MMRSP), 3) Hexamerin with high composition of aromatic amino acids (Arylphorin), 4) Arylphorin-like hexamerin, and 5) Riboflavin-binding hexamerin (RbH). The phylogenetic pattern combined with the comparison of conserved histidine residues in copper binding sites of hexamerins revealed basal position of RbH and the evolutionary pathway in lepidopteran hexamerins. Finally, the induction expression of hexamerins by insecticide, lambda-cyhalothrin, were analyzed, results showed that lambda-cyhalothrin exposure may down-regulate their expression. This study increased the gene number of hexamerin to seven, and reported their expression and structural characterizations, the finding will facilitate the understand of hexamerin in other insects.
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Knockdown of dorsal/relish increases larvae susceptibility to microbes in Spodoptera litura
Yangyang Cai,Zimo Guo,Chunping Ma,Jianya Su 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1
The tobacco cutworm, Spodoptera litura has developed high levels of resistance to frequently-used insecticides, and failures of chemical control has frequently been reported in China. Moreover, the control efficiency of biological control agents was not satisfactory by farmers, and thus the development of novel or combined techniques for such pest control becomes an imperative requirement. In this study the potential application of innate immune-related genes in insect control was investigated. Sldorsal and Slrelish were identified from S. litura. Sldorsals were mainly expressed in epidermal and fatbody of larvae, and Slrelish highly expressed in fatbody and haemolymph of larvae. Their expression characteristic suggests potential roles in defense against fungi or bacteria. The expression of Sldorsal was up-regulated by the challenge of entomogenous fungi Isaria javanica and Beauveria bassiana, however, the expression of Slrelish could only be induced by Escherichia coli challenge. Injection of Sldorsal or Slrelish dsRNA down-regulated the expression level of the corresponding gene and broke specific signaling transduction pathway of innate immunity in S. litura. Knockdown of Sldorsal by RNAi significantly increased the mortality of S. litura larvae caused by I. javanica and B. bassiana, and Slrelish RNAi highly increased the mortality caused by E. coli. Sldorsal and Slrelish are essential genes for innate immunity, their knockdown by RNAi increases S. litura susceptibility to insect pathogens, therefore enhances the mortalities caused by I. javanica, B. bassiana and E. coli respectively.
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Xiangrui Tian,Jidong Liu,Zimu Guo,Bo Hu,Mburu David Kibe,Shiyu Wang,Jianya Su,Qiwei Huang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
Spodoptera exigua has developed very high resistance to pyrethroid insecticides; however, the mode-of-action target of pyrethroids has not been studied, and the mechanism for target resistance remains elusive in this insect. In this study the resistance level of a field collected population from Huizhou, China was assayed with leaf-dip method and further selected in laboratory. The transcripts of voltage-gated sodium channel gene from S. exigua were cloned and analyzed for the structural characteristics, the relative expression levels of transcripts were measured by quantitative RT-PCR. Finally, detection of the point mutations was performed. The bioassay indicated that Huizhou population had developed 309.5-fold resistance to lambda-cyhalothrin, and the selection in laboratory led to the resistant strain (HZ14-sel) with increased resistance of 423-fold. Nine transcripts of sodium channel with 4517–6149 bp nucleotides were identified from S. exigua, among which only one transcript (SeNav4) encode the functional protein with complete domains, and other eight transcripts were observed to lost some transmembrane segments. The L834F mutation in IIS6 corresponding for the typical kdr mutation “L1014F”, was detected in 50% individuals of HZ14-sel strain. These results suggested that lambda-cyhalothrin resistance is associated with point mutations and expression level changes of sodium channel transcripts in HZ14-sel strain.
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