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      • KCI등재

        Effect of Ultrasonic-assisted Ionic Liquid Pretreatment on the Bleachability and Properties of Eucalyptus Kraft Pulp

        Jianmin Peng,Letian Qi,Guihua Yang,Ming He,Yu Xue,Jiuachuan Chen 한국펄프·종이공학회 2019 펄프.종이기술 Vol.51 No.2

        In this work, the application of ionic liquid (IL) and ultrasonic (Ul) treatment on the bleachability and properties of eucalyptus kraft pulp were investigated. The results showed that ILs protected fibers from depolymerization by improving the pulp viscosity. The combination of Ul and IL treatment notably reduced the kappa number and improved tensile index, burst index, tear strength index and folding endurance of pulp. Ul and IL treatment reduced the fines content, decrease the water retention value (WRV) and cause the formation of crosslinks between fibers, especially in Ul+[TEA][HSO4] treatment. The XRD and FT-IR analysis illustrated the combination of Ul and IL treatment disturbed the amorphous region of pulp fiber by increasing of crystallinity and no chemical change appeared in the treatment process. The Ul assisted IL treatment technology will have a promising application due to its high efficiency and environmental protection character.

      • KCI등재후보

        The Relationship of Basketball Participation Motivation, Involvement, Satisfaction, and Adherence among Middle School Students in China

        Peng Qiu,Jianmin Shi,Wang Zuo,Yan Xing 아시아스포츠융합과학회 2023 Asia Pacific Journal of Applied Sport Sciences Vol.4 No.2

        PURPOSE This study aimed to examine the relationship among participation motivation (PM), involvement (IN), satisfaction (SA), and adherence (AD) in middle school basketball participants in China. METHOD Participants were recruited from 10 middle schools in Changchun, Tonghua, Baicheng, Yanbian, and Siping in Jilin Province, China. A total of 800 questionnaires were distributed, and around 793 questionnaires were used for the final analysis. Data were analyzed using SPSS 26 and AMOS 23. RESULT This study determined the PM, IN, SA, and AD of middle school basketball participants through quantitative analysis. Through the establishment and verification of the structural equation model, the following findings were revealed: first, PM directly affects IN. Second, PM directly affects the development of AD. Third, PM directly affects SA. Fourth, IN does not affect SA. IN directly affects the development of AD. Sixth, SA directly affects the development of AD. CONCLUSION To improve the AD of middle school students to participate in basketball, physical education managers and teachers in Jilin Province should first master students' PM and adopt strategies to strengthen it. They must understand students' needs for basketball participation through their IN while improving their SA. This study provides a theoretical reference for Chinese middle school basketball-related researchers. It may provide corresponding methods and strategies for increasing middle school students' basketball AD, thereby further aiding the steady development of the sport in Chinese middle schools.

      • KCI등재

        Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity

        Qizhang Long,Wenwei Zhang,Peng Wang,Wenbiao Shen,Tong Zhou,Nannan Liu,Ren Wang,Ling Jiang,Jiexue Huang,Yihua Wang,Yuqiang Liu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4

        Lipoxygenases (LOXs) are enzymes involved in lipid peroxidation. Here we reported the identification, molecular and functional characterization of the gene encoding rice (Oryza sativa L.) seed LOX3 (sLOX3). Via a map-based cloning strategy we identified Os03g0700400 as the candidate gene encoding sLOX3. Further functional complementary test and biochemical characterization of the recombinant Os03g0700400 protein verified the identification. The sLOX3 gene was highly expressed in roots, moderately in embryos and very weakly in leaves, leaf sheaths and stems. Transient expression experiment (in rice protoplasts) and subsequent laser confocal microscopic analysis demonstrated that the sLOX3 protein was localized into the cytosol. We next showed that overexpression of sLOX3 in a japonica sLOX3-normal rice cultivar, Wuyunjing 7 accelerated the decrease of seed germination ability when the seeds were routinely stored, which demonstrated that sLOX3 had a negative effect on seed longevity (storability). Meanwhile, an increased occurrence of embryo decay was observed in the same transgenic seeds, suggesting that sLOX3 might negatively affect seed longevity by facilitating colonization of particular seed pathogens. Our result forwarded the understanding of the effects of 9-LOX on rice seed longevity.

      • KCI등재

        A putative plastidial adenine nucleotide transporter, BRITTLE1-3, plays an essential role in regulating chloroplast development in rice (Oryza sativa L.)

        Jia Lyu,Yihua Wang,Linglong Liu,Chunming Wang,Yulong Ren,Cheng Peng,Feng Liu,Yunlong Wang,Mei Niu,Di Wang,Ming Zheng,Kunneng Zhou,Shaolu Zhao,Fuqing Wu,Haiyang Wang,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

        Differentiation from proplastids into chloroplasts isa light- and energy-dependent process. How this process isregulated is still poorly understood at the molecular level. We herein report a new putative plastidial adenine nucleotidetransporter, BRITTLE1-3 (referred to as OsBT1-3), encoded bythe rice (Oryza sativa) White Stripe Leaf 2 (WSL2) gene. Loss of OsBT1-3 function results in defective chloroplastbiogenesis, severely reduced photosynthetic efficiency, andfinally a white stripe leaf phenotype in the first four leaves. The expression levels of genes related to chlorophyllbiosynthesis and photosynthesis are drastically reduced,accompanied with over accumulation of reactive oxygenspecies (ROS) in the wsl2 mutant. OsBT1-3 is targeted tothe chloroplasts and it expresses in almost all tissues inplants, especially in young leaves. OsBT1-3 consists of 419amino acids and exhibits features of all mitochondrialcarrier proteins, including a typical transmembrane-spanningdomain and a highly conserved sequence motif designatedas the ‘mitochondrial energy transfer signatures’. Phylogeneticanalysis shows that OsBT1-3 is a putative plastidialadenine nucleotide transporter and is most closely relatedto ZmBT1-2. Together, these observations suggest that thenew putative adenine nucleotide transporter, OsBT1-3,plays an essential role in regulating chloroplast biogenesisand maintenance of ROS homeostasis during rice seedlingde-etiolation.

      • KCI등재

        Green-revertible Chlorina 1 (grc1) is Required for the Biosynthesis of Chlorophyll and the Early Development of Chloroplasts in Rice

        Jieqin Li,Yihua Wang,Juntao Chai,Lihua Wang,Chunming Wang,Wuhua Long,Di Wang,Yunlong Wang,Ming Zheng,Cheng Peng,Mei Niu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.5

        The nuclear genes involved in chloroplast developmentand chlorophyll biosynthesis must be investigated tounderstand their functions in plant growth and development. In this study, we isolated and identified a unique leaf-colormutant of rice with a green-yellow phenotype before thefour-leaf stage and named the mutation green-revertiblechlorina 1 (grc1). The mutants had significantly lower plantheight, number of tillers, and panicle length and headedsignificantly earlier than the wild type. The levels ofchlorophylls, carotenoids, and chlorophyll precursors werealso lower. The mutation in grc1 affected chloroplastultrastructure, particularly thylakoid development. Geneticanalysis indicated that the green-yellow phenotype wascontrolled by a single recessive gene. We mapped the grc1gene to a 32.4-kb region on the long arm of chromosome 6. Through map-based cloning, we identified a 45-bp insertionin the genomic region of LOC_Os06g40080, which encodeda heme oxygenase. Expression of LOC_Os06g40080 wassignificantly down-regulated in the grc1 mutant. Subcellularlocalization showed that this heme oxygenase was localizedin the chloroplast. In summary, we isolated and identified thegene for grc1, which plays an important role in chlorophyllbiosynthesis and chloroplast development in rice.

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