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- 저자 : Jianchun Su (검색결과 1 건)
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Hua Li,Hongjing Dong,Jianchun Su,Bin Yang 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.9
The influence of phenological stages onchemical composition of Scutellariae Radix (SR), theroot of Scutellaria baicalensis Georgi, was investigated. In order to deal with a large quantity of samples, arapid ultra-performance liquid chromatographic (UPLC)was first developed and validated for the simultaneousquantification of five flavonoids, namely baicalin(baicalein-7-O-b-D-glucuronide, BG), wogonoside(wogonin-7-O-b-D-glucuronide, WG), baicalein (BA),wogonin (WO), oroxylin A (OA) in the samples. Goodlinearity was obtained in the range of 0.742–389 ng(r2[0.9999) and satisfactory recoveries were obtained(101.72–104.56 %) with the RSD value below 5.0 %,for all analytes. Also, extraction conditions were optimizedto obtain maximum extractive contents of thefive flavonoids. Content variations of the five activeingredients in 225 samples from three different originswere investigated in five major phenological periods. Itwas found that the effect of phenology on the contentsof the tested five flavonoids was similar in the threeorigins. The contents of flavone O-glycosides, i.e., BGand WG accumulated to the highest level in leafexpansion period, while flavonoid aglycones, i.e., BA, WO and OA appeared a maximum concentration inflowering period. The UPLC method established in thisstudy was rapid and of good accuracy, repeatability andresolution, and hence can assist in the quality control ofSR.
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