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Jennifer Sang-jee Lee,Se-Jeong Kim,최지숙,Min Rye Eom,Heungsoo Shin,Seong Keun Kwon 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.91 No.-
The goal of this study was to investigate the effect of transplantation of an adipose-derived stem cell(ASC) spheroid sheet on the regeneration of ulcerated oral mucosa. The ASC spheroid sheet wasconstructed by seeding three-dimensional (3D) spheroidal ASCs on a temperature-responsive hydrogel,where cell adhesion and spreading were improved by incorporatingfibronectin. The 3D culture of ASCsenhanced their inherent paracrine properties for up to 1 week after transferring to two-dimensional (2D)culture conditions. Expansion of the hydrogel through temperature change promoted effective deliveryof ASCs to the site of injury, and viability was maintained after transferring ASCs to the target substrate. Treatment with the ASC spheroid sheet accelerated wound closure and regeneration of the stratifiedmulti-layered epithelium. The regenerated epithelium not only had structural integrity, but alsodisplayed phenotypic polarization across the mucosa; basal epithelial cells expressed cytokeratin 5,whereas cytokeratin 13-positive cells were located in the supra-basal layers. In addition, by day 7 aftertreatment, the mucosa that received the ASC spheroid sheet had significantly decreased inflammation,similar to that of the normal mucosa. These results demonstrate that the ASC spheroid sheet is aneffective treatment for ulcers in the oral cavity.