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- 저자 : Jayanthi Madhavan (검색결과 1 건)
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Production of nematode free plantlets in Polianthes tuberosa using in vitro culture techniques
Kanchan B. M. Singh,Jayanthi Madhavan,Raghunath Sadhukhan,Shivani Chandra,Uma Rao,Pranab Kumar Mandal 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.5
Tuberose ( Polianthes tuberosa ) cultivation is tremendously aff ected due to Meloidogyne incognita infection. Histologicalstudy of in vitro nematode infected tuberose roots showed that the root infection initiated within 2 days post inoculation(DPI) and root gall formation occurred at 6 DPI indicating established infection on the roots. The life cycle of M. incognitain tuberose roots completed within 45 DPI evident by the formation of large number of eggs. Our study established in vitromethods like shoot tip culture and callus mediated regeneration of tubers collected from nematode infection fi elds to obtaincompletely nematode free plantlets. Tubers of diff erent varieties produced multiple shoot bud on Murashige and Skoog (MS)media containing 4 mg L −1 6-benzylaminopurine (BAP) and 0.1 mg L −1 α-naphthaleneacetic acid (NAA). Maximum numbersof plantlets were obtained for Calcutta Single (14.4 ± 2.0 per plant). Embryogenic callus was induced on MS medium supplementedwith altered concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), NAA and BAP from leaf, fl ower, root andtuber explant. The maximum callus induction was obtained on MS containing 1 mg L −1 2,4-D, 1 mg L −1 NAA and 0.5 mg L −1BAP (100%) and 1 mg L −1 2,4-D and 2.25 mg L −1 BAP (96.7%). Regeneration of tuber callus was achieved on MS with0.5 mg L −1 Kinetin (KIN) within 3 weeks. Plantlets were rooted on ½ MS with 0.5 mg L −1 Indole-3-butyric acid for 25 days. The in vitro regeneration protocol developed can thus be used for producing disease free plantlets for mass propagation.
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