포인섿치아의 細胞培養硏究

J. L. Won(元種樂) 한국육종학회 1984 한국육종학회지 Vol.16 No.3

For the induced mutation using cultured cells of poinsettia, effective nutrition medium for cell culture, cell survival and proliferation, and radiosensitivity were investigated. Hormone concentration of CPA 0.2 and BAP 0.2 ppm in MS basal medium was effective for callus formation and single cell production, showing cell survival rate of 74% at 15 days after culture. Cell proliferation in batch culture showed logarithmic growth phase from 5 to 14 days and stationary phase in 15-18 days. LD50% of microcluster by radiation treatment was 30 GY. Meristematic single cells originated from microcluster had higher viability and it was easy to regenerate whole plant for practical utilization.

放射線에 依한 水稻의 可視的 突然變異의 出現率에 關한 硏究

J.L. WON(元鐘樂),S.H. KWON(權臣漢) 한국육종학회 1977 한국육종학회지 Vol.9 No.3

Frequency of macro-visible mutations such as early heading, short culm, leaf and plant type etc. was compared with that of chlorophyll mutions in X-irradiated rice seeds with 25kR. Various morphological mutation types of different M₁ panicles and segregation ratio of mutant types in M₂ generation were investigated. Rate of macro-visible mutation was much higher than that of chlorphyll mutation and the majority of mutation type was early heading and short culmed types among the macro-visible mutations. Since the same types of morphological mutation were found in different panicles of single M₁ plant, one panicle selection per M₁ plant will be enough for raising M₂ progenies in mutation breeding. Consequently, estimation of number of initial cells in the seed embryo by means of M₂ segregation ratio will be reconsidered.

放射線을 處理한 大麥 第二世代에서의 突然變異 出現樣相

J.L. WON(元種樂),Y.O. KO(高永玉) 한국육종학회 1983 한국육종학회지 Vol.15 No.1

In M₂ generation of barley treated with radiation, mutation frequency, segregation pattern, radiation damage and mutation rate, and sector size were investigated. Frequency of chlorophyll mutation with albina and xantha type was 8.8% in Mz family and 0.89% in M₂ plant basis. Chlorophyll mutations in M₂ seedling were segregated with fit type to 3:1, recessive excessive and unidentified type. Mutation rate of albina was higher with increase of number of seeds per M₁ spike and in xantha type no tendency was found between mutation rate and seed numbers per M₁ spike. Sector size in mutated plant with albina was average 14. 1% and 6.0% in xantha type.

放射線에 依한 水稻 可視的 突然變異의 M₃ 世代의 分離 및 劣性不足

J. L. WON(元鍾樂),S. H. KWON(權臣漢) 한국육종학회 1979 한국육종학회지 Vol.11 No.1

In order to obtain fundamental information for mutation breeding of cereal crops, segregation and recessive deficit in M₃ generation of short culm, early and late heading mutant lines were investigated in rice. About 32% of the total mutant lines selected from M₂ generation was segregated in M₃ generation, and among them segregated lines of short culm, early and late heading mutant lines were 31, 22, and 43%, respectively. Average segregation ratio in M₃ segregating lines of short culm mutants was 0.17, indicating considerable recessive deficit of mutant from. the expected ratio of 0.25, and the tendency of segregation ratio of M₃ was not in confirmity with the trend of M₂ generation. The ratio of fixed lines vs segregating lines in, M₂ basis was deviated frori the expected ratio of 1 : 2 with the deficit or excessive.

대두내륭성계통의 선발에 관한 연구

권신한,원종락,Kwon, S.H.,Won. J.L. 한국작물학회 1979 한국작물학회지 Vol.24 No.3

In order to screen the shade tolerance soybean lines, several important agronomic characters were studied after shade treatment at various growth stages. A severe influence of light reduction on agronomic characters found during the late flowering to pod filling period, and the response to shading was significantly different among the lines tested. Examining of pods number per plant after shade treatment during pod filling stage would be most effective method in the screening for shade tolerance soybean lines.

水稻 突然變異系統의 세가지 形質의 遺傳硏究

S.H. KWON(權臣漢),J.L. WON(元鍾樂) 한국육종학회 1978 한국육종학회지 Vol.10 No.2

Rice mutant having brown spot color on lemma, earliness and long culmness was obtained from a population of X-irradiated Jinheung variety. To Analyse gene controlling the mutant character and to verify genetical relationships among the three characters, crossing between mutant and mother variety was made. Segregation in F₂ of each character revealed that the charactors were controlled by monogenic recessive gene and the three characters were inherited simultaneously. This implies that the mode of inheritance might be resulted by either pleiotropism or close linkage relationship of the three characters, and a definite answer will become clear by further experiments.

감마線을 處理한 大豆 第二世代에서의 突然變異出現率

S.H. KWON(權臣漢),J.L. WON(元鍾樂),H.S. SONG(宋禧燮) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

In order to investigate the radiosensitivity in M₁ and frequency of mutants at seedling stage in M₂ population, the air dried seeds of three varieties were irradiated with gamma-ray doses ranged 10~50kR. The fifty percent decrease due to the radiation effects in plant height at 14 days, survival rate at 40 days after seeding, and number of pods per plant at harvest was ranged from 20 to 30 kR and their varietal differences were not found. Frequency of chlorophyll deficient mutation of leaf was 4~7% in M₁ plant bais and 2~4% in M₂ seedling basis within 20~30kR, and significant differences were not, found among the varieties. Frequency of stem color mutation in Korean native line, KAS 552-4 was higher as compared to that of chlorophyll deficient mutation and the rate was 25% and 7% in M₁ and M₂ basis, respectively. The purpose of experiment was to investigate the radiosensitivity in M₁ generation and frequency of mutants at seedling stage in M₂ population of soybean. The air dried seeds of three varieties, Chungbuk-Baik, KAS 552-4 and CB-27 were irradiated with gamma-ray doses of 10, 20, 30, 40 and 50kR. The results obtained are summarized as follows: 1. The fifty percent decrease due to the radiation effects in plant height at 14 days after seeding, survival rate at 40 days after seeding, and number of pods per plant at harvest was ranged from 20 to 30kR and their varietal differences were not found. 2. Mutant types at M₂ seedling stage were classified into chlorophyll deficiency of leaf, stem color at cotyledon stage, rosette type, twin stem, narrow leaf and multi-leaflets types. 3. Frequency of chlorophyll deficient mutation of leaf with xantha, chlorina, chlorotica and maculata type was 4~7% in M₁ plant basis and 2~4% in M₂ seedling basis within 20~30kR, and significant difference were not found among the varieties employed 4. Frequency of green stem color mutation in Korean native line, KAS 552-4 with purple stem at cotyledon stage was higher as compared to that of chlorophyll deficient mutation and the rate was about 25% and 7% in M₁ and M₂ basis, respectively.

감마線處理에 依한 大豆의 可視的 形態突然變異의 出現率

S.H. KWON(權臣漢),J.L. WON(元鐘樂),H.S. SONG(宋禧燮) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

In order to investigate the frequency of chlorophyll and visible-macro mutations in M₂ population, the air-dried seeds were irradiated with 30kR of gamma-rays. The frequency of chlorophyll-deficient mutation of leaf was about 11% on M₁ plant basis and 1.8% in M₂ seedlings. The rate of visible-macro mutations, such as early and late flowering, shortness, erectoid, rosette, rugose, flower and pubscence color, leaf specks and yellowish-leaf type was about 15.430 and 3.7% on M1 and Ma plant basis, respectively, and the majority of visible macro-mutation types was shortness, early and late flowering, flower color, narrow leaf and rugose types. The mutation rate of two and three characters occurred coincidently in a M₂ plant was about 17.4 based on M₁ mutation plant. Segregation rate of mutants in M₂ averaged about 15% in chlorophyll mutations and 32% in visible-macro mutation types.

무궁화 突然變異 育種을 위한 放射線感受性과 Chimera에 關한 硏究

S.H. KWON(權臣韓),J.L. WON(元鍾樂) 한국육종학회 1980 한국육종학회지 Vol.12 No.1

Radiosensitivity of gamma-irradiated Hibiscus syriacus and chimera formation were investigated. The lethal dose 50 percent with respect to seeding and cuttings was 15kR and 2~3kR respectively, chlorophyll mutation rate of seeds irradiated with 15kR being about 13 percent. The degree of chimeric leaf mutants from the buds by radiation treatment depends on the bud position of the branch. Buds of the middle part of V₁ branch seemed to be more multi-cellular condition than the upper and low part when irradiation was made. It is assumed that at least two primordia of V₂ branch were already differentiated from the V₁ branch in Hibiscus syriacus plant.

大豆의 放射線感受性과 突然變異率

S.H. KWON(權臣漢),J.L. WON(元鍾樂),J.R. KIM(金在利) 한국육종학회 1980 한국육종학회지 Vol.12 No.3

In order to obtain the basic information for the soybean mutation breeding, the seeds were treated with various gamma-ray doses of 10, 20, 30, 40, 50kR, and the response to different characters at seeding and harvest stage and mutation frequency using chlorophyll deficient mutation were investigated. Plant height, number of pods per plant and survival rate seemed to be appropriate characters to decide the radiosensitivity, and radiation doses of 20~30kR are recommended for mutation breeding. Chlorophyll deficient mutations were classified with xantha, chlorina, chlorotica and maculata types, and their frequency was 1.46 and 2.44% in M₂ plant basis at 20 and 30kR, respectively.