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- 저자 : Itharat, Arunporn (검색결과 4 건)
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Yaowapha Jirakiattikul,Panumart Rithichai,Papichaya Kwanthong,Arunporn Itharat 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.4
This study investigated jasmonic acid (JA) elicitation of the callus of two roselle accessions: HS003 (orange-red calyx) and HS005 (dark-red calyx). The goal was to identify the optimal exposure period for production of total anthocyanin, phenolics, and flavonoids and for enhancing DPPH radical scavenging. Dark-red callus was cultured on Murashige and Skoog (MS) medium supplemented with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 4.44 µM 6-benzyladenine, combined with 0 and 50 µM JA for treatment of HS003, and with 0 and 100 µM JA for treatment of HS005. Elicitation periods of 7, 14, 21, and 28 days were compared. Significant increases in both accumulation of bioactive compounds and enhancement of antioxidant activity were observed. In the case of HS003, the optimal exposure periods were 21 days for enhancement of total anthocyanin accumulation (1.48 ± 0.07 mg cyd-3-glu L−1, or 3.70 times that of the control) and 14 days for enhancement of total phenolic and flavonoid contents (114.72 ± 6.63 mg GAE g−1 dry extract and 106.15 ± 9.06 mg CE g−1 dry extract, or 6.89 and 18.59 times that of the control). In the case of HS005, elicitation with 100 µM JA for 28 days yielded the greatest increase in total anthocyanin (0.86 ± 0.06 mg cyd-3-glu L−1, or 6.61 times that of the control), phenolics (48.01 ± 2.47 mg GAE g−1 dry extract, or 3.37 times that of the control), and flavonoids (21.41 ± 1.89 mg CE g−1 dry extract, or 3.30 times that of the control). At all periods, and for both accessions, JA elicitation increased DPPH radical scavenging, compared with the control. Our results demonstrated that the dark-red callus of roselle accumulated greater amounts of bioactive compounds and exhibited enhanced antioxidant activity, following elicitation with JA for 14–21 days in the case of HS003 and 28 days in the case of HS005.
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Thitiorul, Sumon,Ratanavalachai, Treetip,Tanuchit, Sermkiat,Itharat, Arunporn,Sakpakdeejaroen, Intouch Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4
In Thai traditional medicine, Plumbago indica or Jetamul-Pleung-Dang in Thai is known to have health benefit especially for anti-inflammatory, antibacterial, and antitumor activities. However, the mechanisms of its action are still uncertain. One of which might be genotoxic effects. In the present study, we investigated the genotoxicity of an ethanolic extract of Plumbago indica root (EEPIR) by sister chromatid exchange (SCE) assay in human lymphocytes. Results have shown that all treatments with EEPIR ($12.5-100{\mu}g/ml$) could induce cell cycle delay as shown by significant increase in the number of metaphase cells in the first cell cycle but neither in the second nor the third cell cycle. Only at concentrations of 25, 50, and $100{\mu}g/ml$ were SCE levels significantly increased above that of the control (p<0.05). EEPIR at a concentration of $500{\mu}g/ml$ induced cell death as few mitotic cells were shown. Accordingly, EEPIR ($25-100{\mu}g/ml$) is genotoxic in human lymphocytes and cytotoxic at concentrations of ${\geq}500{\mu}g/ml$ in vitro. Therefore, these activities of the EEPIR could serve its potential therapeutic effects, especially as an anticancer agent. Further study of EEPIR in vivo is now needed to support this in vitro evidence.
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Plengsuriyakarn, Tullayakorn,Viyanant, Vithoon,Eursitthichai, Veerachai,Tesana, Smarn,Chaijaroenkul, Wanna,Itharat, Arunporn,Na-Bangchang, Kesara Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Cholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells of the bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activity of a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activity against a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was performed by DNA fragmentation assay and induction of genes that may be involved in the resistance of CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. To investigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/DMN)-induced CCA hamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily or every alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with 5-fluorouracil (positive control) or distilled water (untreated control). Median $IC_{50}$ (concentration that inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolic extract of ginger were 10.95, 53.15, and $27.86{\mu}g/ml$, respectively. More than ten DNA fragments were visualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed following exposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of any significant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage. The survival time and survival rate of the CCA-bearing hamsters were significantly prolonged compared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivo studies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCA with the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved in conferring resistance of CCA to the ginger extract.
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In vitro cytotoxic and antioxidant activities of Pikut Trichinthalamaga remedy
Bhanuz Dechayont,Chayaporn Limpichai,Kornrawee Kornwisitwathin,Nitra Nuengchamnong,Arunporn Itharat 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.3
The Pikut Trichinthalamaga remedy appears in ancient Thai pharmacy scripture, as a purgative, and includes Rhubarb (Rheum palmatum L.), Myrabulan fruit (Terminalia chebula Retz.), and Gamboge (Garcinia handuryi Hook F.). We investigated the cytotoxic properties of the herbal extracts using the SRB assay in human colon adenocarcinoma cell lines (LS174T and SW480) and normal lung fibroblast cell (MRC-5) as well as their antioxidant activities by chemical-(ABTS assay) and cell-based assays [nitric oxide (NO) assay]. The active fractions were identified using high-performance liquid chromatography coupled to electrospray ionisation and quadruple time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). The ethanolic extract of Pikut Trichinthalamaga remedy had a cytotoxic effect on LS174T and SW 480 cells (IC50 = 0.39 and 0.51 μg/ml, respectively). The ABTS assay showed that the water extract of the myrabulan fruit had the highest activity (EC50 = 5.69 μg/ml) while the NO assay showed that the ethanolic extract of Pikut Trichinthalamaga remedy had the most potent inhibitory activity on NO production (EC50 = 0.62 μg/ml). After liquid–liquid partitioning sequence, the chloroform fraction had the highest cytotoxicity. Emodin, forbesione, gaudichaudionic acid and gaudichaudionol were identified in this extract. These results support the further investigation of the Pikut Trichinthalamaga remedy as a potential anticancer drug.
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