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RISS 인기검색어
- 검색키워드
- 저자 : Iruthayasamy Johnson (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Lim, Tae-Heon,Johnson, Iruthayasamy,Cha, Byeong-Jin The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.4
To characterize benzimidazole-resistant and -sensitive Monilinia fructicola populations, the fungal isolates were obtained from peach plants showing brown rot and bloosom blight. Benzimidazole-sensitive isolates did not grow on potato dextrose agar(PDA) amended with $\geq1.0{\mu}g$ active ingredient(a.i.)/ml of the fungicides. However, benzimidazole-resistant isolates grew on PDA regardless of the tested concentrations of fungicides. Benzimidazole-resistant isolates did not grow on diethofencarb-PDA, but sensitive isolates grew on the same PDA. In the nucleotide sequences of $\beta$-tubulin gene, only codon 198(GAG: glutamic acid), a target site for benzimidazole, was replaced with GCG(alanine) in all of the resistant isolates, and this substitution seems to play an important role in the development of resistance. Other interesting codons such as 165(GCT), 200(TTC), and 241(GCT) were not changed among the isolates. Benzimidazole-resistant and -sensitive isolates were clustered clearly in random amplified polymerphic DNA analyses and the results revealed that low levels of genetic diversity between benzimidazole-sensitive and -resistant isolates of M. fructicola in the investigated regions.
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Effects of Streptomyces griseofuscus 200401 on Melon Powdery Mildew inGreenhouse
임태헌,조정상,강상재,Iruthayasamy Johnson,차병진,최용화 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.1
The fermentation broth (FB) of Streptomyces griseofuscus 200401 isolated from non-farming soil showed antagonistic activity against powdery mildew fungus both in melon leaf/seedling assay and in field trials. The FB of S. griseofuscus 200401 was tested at different concentrations. In primary test, the control value of 2- fold diluted FB of S. griseofuscus 200401, compared to control, reached to 82.8%. The protective activity recorded 80.5% in 2-fold dilution of FB. The effect was reduced to 28.9% in high dilution (100-fold) treatment. The curative effect was relatively lower than protective activity. In field test, the antifungal activities of S. griseofuscus 200401 remained low in blocks sprayed with 100-fold diluted FB and the control values were 15.2 and 15.9% in 2005 and 2006, respectively. However, the activities were as high as 65% and 67.3% in the blocks treated with 2-fold dilution of FB during the same period.
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Tae Heon Lim,Iruthayasamy Johnson,차병진 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.4
To characterize benzimidazole-resistant and -sensitive Monilinia fructicola populations, the fungal isolates were obtained from peach plants showing brown rot and bloosom blight. Benzimidazole-sensitive isolates did not grow on potato dextrose agar (PDA) amended with ≥1.0 μg active ingredient (a.i.)/ml of the fungicides. However, benzimidazole-resistant isolates grew on PDA regardless of the tested concentrations of fungicides. Benzimidazole-resistant isolates did not grow on diethofencarb-PDA, but sensitive isolates grew on the same PDA. In the nucleotide sequences of β-tubulin gene, only codon 198 (GAG: glutamic acid), a target site for benzimidazole, was replaced with GCG (alanine) in all of the resistant isolates, and this substitution seems to play an important role in the development of resistance. Other interesting codons such as 165 (GCT), 200 (TTC), and 241 (GCT) were not changed among the isolates. Benzimidazole-resistant and -sensitive isolates were clustered clearly in random amplified polymerphic DNA analyses and the results revealed that low levels of genetic diversity between benzimidazole-sensitive and -resistant isolates of M. fructicola in the investigated regions.
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Effects of Streptomyces griseofuscus 200401 on Melon Powdery Mildew in Greenhouse
Lim, Tae-Heon,Cho, Jeong-Sang,Kang, Sang-Jae,Johnson, Iruthayasamy,Cha, Byeong-Jin,Choi, Yong-Hwa The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.1
The fermentation broth (FB) of Streptomyces griseofuscus 200401 isolated from non-farming soil showed antagonistic activity against powdery mildew fungus both in melon leaf/seedling assay and in field trials. The FB of S. griseofuscus 200401 was tested at different concentrations. In primary test, the control value of 2-fold diluted FB of S. griseofuscus 200401, compared to control, reached to 82.8%. The protective activity recorded 80.5% in 2-fold dilution of FB. The effect was reduced to 28.9% in high dilution (100-fold) treatment. The curative effect was relatively lower than protective activity. In field test, the antifungal activities of S. griseofuscus 200401 remained low in blocks sprayed with 100-fold diluted FB and the control values were 15.2 and 15.9% in 2005 and 2006, respectively. However, the activities were as high as 65% and 67.3% in the blocks treated with 2-fold dilution of FB during the same period.
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