http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Inke Van Marcke (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Genomic stability in Nicotiana plants upon silencing of the mismatch repair gene MSH2
Marcke, Inke Van,Angenon, Geert 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
The Mismatch Repair (MMR) system is a highly conserved pathway for the maintenance of genomic stability in many organisms. In plants, this is particularly important because of the lack of a reserved germline. Suppression of MMR leads to an accumulation of random mutations in the genome over successive generations, and thus maximizes genetic diversity. MMR deficiency has been shown to be a useful technique in plant breeding, complementary to chemical or physical mutagenesis. We have developed an artificial microRNA (amiRNA) targeting the MSH2 gene, which is generally applicable in Solanaceae. Two amiRNA precursors were inserted in a transformation vector, under the control of the CaMV 35S promoter and the meiosis active AtDMC1 promoter, respectively. Introduction of this amiRNA construct in Nicotiana tabacum and N. plumbaginifolia reduced the MSH2 transcript levels to 20-30 %. Morphological and developmental abnormalities and plants with white sectors on the first pair of leaves or on the cotyledons (referred to as 'chimeric albinos') appeared in the transformed Nicotiana lines at higher frequencies than in the control lines. Also, some plants which show an increased tolerance for the herbicide chlorsulfuron were found. However, the mutant phenotypes were not transmitted to subsequent generations. We conclude that the designed amiRNA was capable of suppressing the MSH2 activity, which caused the occurrence of somatic mutations. Apparently, the silencing of MSH2 was not strong enough in the germline to cause inheritable mutations.
-
Genomic stability in Nicotiana plants upon silencing of the mismatch repair gene MSH2
Inke Van Marcke,Geert Angenon 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
The Mismatch Repair (MMR) system is a highly conserved pathway for the maintenance of genomic stability in many organisms. In plants, this is particularly important because of the lack of a reserved germline. Suppression of MMR leads to an accumulation of random mutations in the genome over successive generations, and thus maximizes genetic diversity. MMR deficiency has been shown to be a useful technique in plant breeding, complementary to chemical or physical mutagenesis. We have developed an artificial microRNA (amiRNA) targeting the MSH2 gene, which is generally applicable in Solanaceae. Two amiRNA precursors were inserted in a transformation vector, under the control of the CaMV 35S promoter and the meiosis active AtDMC1 promoter, respectively. Introduction of this amiRNA construct in Nicotiana tabacum and N. plumbaginifolia reduced the MSH2 transcript levels to 20–30 %. Morphological anddevelopmental abnormalities and plants with white sectors on the first pair of leaves or on the cotyledons (referred to as ‘chimeric albinos’) appeared in the transformed Nicotiana lines at higher frequencies than in the control lines. Also, some plants which show an increased tolerance for the herbicide chlorsulfuron were found. However, themutant phenotypes were not transmitted to subsequent generations. We conclude that the designed amiRNA was capable of suppressing the MSH2 activity, which causedthe occurrence of somatic mutations. Apparently, the silencing of MSH2 was not strong enough in the germline to cause inheritable mutations.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
