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Ilavenil Soundharrajan,Palaniselvam Kuppusamy,Hyun Jeong Lee,Ki Choon Choi 한국초지조사료학회 2019 한국초지조사료학회 학술대회논문집 Vol.2019 No.06
Lactic acid bacteria (LAB) were isolated and analysed its fermentation ability in triticale powder at different moisture levels. Furthermore, the antibacterial activity of fermented silage extract against cattle pathogens was also studied. The isolated strains were P. pentosaceus (TC48) and L. brevis (TC50) that confirmed based on biochemical and 16srRNA sequences methods. Extract from LAB fermented silage showed higher antibacterial activity (inhibition zone diameters: 18~24.2 mm) against E. coli P. aeruoginosa, S. aureus and E. Fecalis than the non-inoculated silage extract. TC48 and TC52 strains exhibited high tolerance to artificial gastric, duodenal and intestinal fluids. In summary, lactic acid bacteria mediated fermentation of triticale silage extract showed great antibacterial activity with significant probiotic characteristics might be an effective and safe way to provide new strategies for reducing the incidence of pathogenic bacteria associated diseases in animals.
Beneficial Effects of Lactic Acid Bacteria Inoculation on Oat Based Silage in South Korea
Ilavenil, Soundharrajan,Srigopalram, Srisesharam,Park, Hyung Soo,Kim, Won Ho,Lee, Kyung Dong,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2015 한국초지조사료학회지 Vol.35 No.3
The objective of the study was to measure the beneficial effects of lactic acid bacteria (LAB) inoculation on the nutritive value of oat silage collected from thirteen regions in the Republic of Korea. The contents of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude ash (CA) were slightly lower in LAB inoculated silage when compared with the control silage, whereas inoculation of LAB resulted in increased total digestible nutrient (TDN). Higher number of LAB, but lower count of yeast and fungi indicated the effectiveness of the LAB inoculation on oat silage fermentation. LAB inoculation resulted in low pH silage, which may prevent undesirable microbial growth. The LAB inoculation promoted lactic acid dominant fermentation with marginal levels of acetic acid and butyric acid in oat silage. These data suggest that the LAB inoculation may preserve oat silage at better quality for ruminant animal production.
Ilavenil, Soundharrajan,Kim, Da Hye,Srigopalram, Srisesharam,Kuppusamy, Palaniselvam,Valan Arasu, Mariadhas,Lee, Kyung Dong,Lee, Jung Chae,Song, Yeon Hee,Jeong, Young-Il,Choi, Ki Choon ELSEVIER SCIENCE B.V.; AMSTERDAM 2017 JOURNAL OF FUNCTIONAL FOODS Vol.37 No.-
<P><B>Abstract</B></P> <P>Ferulic acid (FA), a ubiquitous natural phenolic component found in many plants and fruits, has a wide range of biomedical applications. However, action mechanism of FA involved in lipid accumulation remains unclear. In this study, lipid accumulation and changes in expression levels of genes and proteins associated with adipocyte differentiation were investigated. Oil red O staining and glycerol accumulation assay revealed that FA decreased lipid accumulation in cells. FA downregulated expression levels of C/EBP-β, C/EBP-α, PPAR-γ, and SREBP-1, but upregulated expression levels of p38MAPK, p44/42 (Erk 1/2), and AMPK-α phosphorylation in 3T3-L1 cells. FA effects on high fat diet-induced (HFD) obese mice were also investigated. FA lowered HFD-induced body weight gain of obese mice without affecting regular food intake. FA reduced serum levels of total cholesterol and triglycerides in HFD obese mice. Similar to results of <I>in vitro</I> study, FA inhibited adipogenesis and lipid accumulation via downregulating PPAR-γ while upregulating p38MAPK, p44/42 (Erk 1/2), and AMPK-α phosphorylation in Swiss albino mice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ferulic acid (FA) extracted from Italian ryegrass and identified by HPLC. </LI> <LI> FA inhibits adipocyte differentiation via downregulation of key factors-<I>invitro</I>. </LI> <LI> FA reduced body weight gain in HFD induced obese mice-<I>in-Vivo</I>. </LI> <LI> FA activates p38MAPK, p44/42 (Erk 1/2) and AMPK-α protein expression. </LI> <LI> FA is a potent dietary source for preventing risk of obesity and their related disorders. </LI> </UL> </P>
Lactic Acid Bacteria Mixture as Inoculants on Low Moisture Italian Ryegrass Silage Fermentation
Soundharrajan, Ilavenil,Kuppusamy, Palaniselvam,Park, Hyung Soo,Kim, Ji Hea,Kim, Won Ho,Jung, Jeong Sung,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2019 한국초지조사료학회지 Vol.39 No.3
The effects of lactic acid bacteria (LAB) mixtures on low moisture Italian ryegrass (IRG) silage fermentation was evaluated in field conditions. The experiment was categorized into two groups: Un-inoculated (Control) and Inoculated with LAB mixture for four storage periods (45, 90, 180, and 365 days, respectively). Silage inoculated with the LAB mixture had the lowest pH with highest lactic acid production than the control from beginning at 45-365 days at all moistures. Higher LAB counts were observed in inoculated silages than the control silages at whole experimental periods. It is a key reason for the rapid acidification and higher lactic acid production in silages during the storage periods. Overall results suggest that an adding of LAB mixture had positive effects on the increasing aerobic stability of silage and preserved its quality for an extended duration.
Improvement of Orchardgrass (Dactylis glomerata L.) Silage Quality by Lactic Acid Bacteria
Soundharrajan Ilavenil,Karnan Muthusamy,Jeong Sung Jung,Bae Hun Lee,Hyung Soo Park,Ki Choon Choi 한국초지조사료학회 2021 한국초지조사료학회지 Vol.41 No.4
In the current study, lactic lactic acid bacteria (LAB) Lactobacillus plantarum and Pediococcus pentosaceus were used as a mixed additive for the production of Orchardgrass silage by ensiled method and nutritional change fermentation ability and microbial content of experimental silages. The addition of LAB to Orchardgrass during ensiling process rapidly reduced the pH of the silages than the non-inoculated silages. In addition, the lactic and acetic acid content of silage was increased by LAB strains than the non-inoculated silages whereas butyric acid content was reduced in silage treated with LAB. A microbiological study revealed that higher LAB but lower yeast counts were observed in inoculated silages compared to non-inoculated silage. Overall data suggested that the addition of LAB stains could have ability to induce the fermentation process and improve the silage quality via increasing lactic acid and decreasing undesirable microbes.
Soundharrajan Ilavenil,Jeong Sung Jung,최기춘 한국초지조사료학회 2023 한국초지조사료학회지 Vol.43 No.3
It is widely known that forage corn is one of the most important crops in the production of silage and green chops during the summer months. In this study, the characteristics and the productivity of Kwangpyeongok, AGR 41, and Nero IT corn cultivars were evaluated in the regions of Jeju and Cheonan, Korea between 2021 and 2022. In each year, different corn cultivars were sown in different parts of the country on April 7 in Jeju and May 3 in Cheonan. The yield of three cultivars, Kwangpyeongok, AGR 41, and Nero IT were measured at the yellow-ripening stage, including the leaves and stems (Stover) and corn grain. Kwangpyeongok stover and total dry matter (DM) yields were slightly higher in Cheonan than in Jeju over the past two years. The quantity of corn cultivated in all cultivated areas was similar. The average yield of AGR 41, corn quantity and total DM were slightly higher in Jeju than in Cheonan. In terms of stover yield, and total dry matter content of the Nero IT cultivar cultivated in Jeju had a slightly higher yield than the cultivar cultivated in Cheonan. Based on this study, all cultivars can show some variations but not too much in terms of growth productivity and distinct characteristics depending on their location. Overall, AGR 41, and Nero IT cultivars in Jeju exhibit slightly higher levels of productivity than the same cultivars in Cheonan.