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Cheong, Eun-Ju,Hurtt, Suzanne,Salih, Sarbagh,Li, Ruhui The Plant Resources Society of Korea 2010 한국자원식물학회지 Vol.23 No.3
In vitro elimination of Sweet potato leaf curl virus (SPLCV) from infected sweet potato is difficult due to low number of virus-free plants obtained from meristem tip culture and long growth period required for the virus detection. In this study, efficient production of the SPLCV-free sweet potato by in vitro therapy coupled with a PCR assay for virus detection was investigated. Infected shoots cultured on Murashige and Skoog medium were treated at three different temperatures for 7 weeks followed by meristem tip culture on the medium with or without ribavirin at 50 mg/L. The regenerated plantlets were tested for virus infection by a PCR assay. The results showed that the both heat- and cold-treatments, and addition of the ribavirin did not have significant effect on efficiency of the virus elimination. The meristem size, however, greatly affected the survival rate. Meristems sized over 0.4 mm survived better than smaller ones (0.2-0.3 mm). The PCR assay was approved to be a rapid, sensitive and reliable for the SPLCV detection in regenerated plantlets. Therefore, combination of cultivating meristem tips sized 0.4-0.5 mm on the medium at $22^{\circ}C$ without ribavirin and detection of SPLCV in the regenerated plantlets by the PCR assay was an efficient system for the SPLCV elimination from infected sweet potato.
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Jungclaus, Johann H.,Bard, Edouard,Baroni, Mé,lanie,Braconnot, Pascale,Cao, Jian,Chini, Louise P.,Egorova, Tania,Evans, Michael,Gonzá,lez-Rouco, J. Fidel,Goosse, Hugues,Hurtt, George C.,Jo Copernicus GmbH 2017 Geoscientific model development Vol.10 No.11
<P><p><strong>Abstract.</strong> The pre-industrial millennium is among the periods selected by the Paleoclimate Model Intercomparison Project (PMIP) for experiments contributing to the sixth phase of the Coupled Model Intercomparison Project (CMIP6) and the fourth phase of the PMIP (PMIP4). The <i>past1000</i> transient simulations serve to investigate the response to (mainly) natural forcing under background conditions not too different from today, and to discriminate between forced and internally generated variability on interannual to centennial timescales. This paper describes the motivation and the experimental set-ups for the PMIP4-CMIP6 <i>past1000</i> simulations, and discusses the forcing agents orbital, solar, volcanic, and land use/land cover changes, and variations in greenhouse gas concentrations. The <i>past1000</i> simulations covering the pre-industrial millennium from 850 Common Era (CE) to 1849<span class='thinspace'></span>CE have to be complemented by <i>historical</i> simulations (1850 to 2014<span class='thinspace'></span>CE) following the CMIP6 protocol. The external forcings for the <i>past1000</i> experiments have been adapted to provide a seamless transition across these time periods. Protocols for the <i>past1000</i> simulations have been divided into three tiers. A default forcing data set has been defined for the Tier 1 (the CMIP6 <i>past1000</i>) experiment. However, the PMIP community has maintained the flexibility to conduct coordinated sensitivity experiments to explore uncertainty in forcing reconstructions as well as parameter uncertainty in dedicated Tier 2 simulations. Additional experiments (Tier 3) are defined to foster collaborative model experiments focusing on the early instrumental period and to extend the temporal range and the scope of the simulations. This paper outlines current and future research foci and common analyses for collaborative work between the PMIP and the observational communities (reconstructions, instrumental data).</p> </P>
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