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RISS 인기검색어
- 검색키워드
- 저자 : Huiming Zeng (검색결과 3 건)
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Tianxiu Zhong,Huiming Zeng,Liebao Han,Lijuan Zhang,Shanshan Sun 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.5
Drought resistance is increased in plants by theabsence of the hormone gibberellic acid (GA) or by a lackof GA sensitivity. We studied the effects of tissue-specificreduction in GA levels on drought tolerance, on recoveryfrom drought stress, and on primary and secondary growthusing transgenic tobacco plants expressing the GA-inactivatinggene PtGA2ox1 (GA 2-oxidase) specifically inleaves, stems, or roots. Localized reduction of bioactiveGA1 levels was achieved by tissue-specific expression ofthe PtGA2ox1 gene in leaves using the rbcs promoter (LDplants), in roots using the TobRB7 promoter (RD plants),and in stems using the LMX5 promoter (SD plants). Inresponse to drought stress, all transgenic tobacco plantsexhibited reduced primary and secondary growth andincreased drought tolerance with a corresponding reductionin malondialdehyde levels, higher relative water content,increased proline and sugar content, and elevated peroxidase,superoxide dismutase, and catalase activities relativeto wild-type plants. The highest level of drought toleranceand the most rapid recovery from stress was achieved bylocalized reduction of GA1 in the roots of the RD transgenicplants. In addition, although the total bioactive GA1content in RD and LD plants was essentially identical, theheights of LD plants were significantly greater and droughttolerance was significantly less than in RD plants. It ispossible that the site of gibberellin-related gene expressionplays an important role in the balance between growth anddrought tolerance.
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Gene expression involved in dark-induced leaf senescence in zoysiagrass (Zoysia japonica)
Xiaoxia Cheng,Xiaomei Dai,Huiming Zeng,Yunxia Li,Wei Tang,Liebao Han 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4
Zoysiagrass (Zoysia japonica) is one of the important turfgrass species. Extending green period of zoysiagrass via delaying leaf senescence will make this species have more potential in the turfgrass industry. In this study, we found that zoysiagrass seedlings treated with GA3 could delay the leaf senescence induced by darkness. To study expression of genes responsive to staying green in zoysiagrass, suppression subtractive hybridization (SSH) was used to identify differentially expressed genes between non-GA3-treated and GA3-treated seedlings subjected to darkness. A total of 307 ESTs were generated, of which 226 ESTs clustered into 54 contigs and 81 were singlets. Differentially expressed genes selected by subtractions were classified into six categories according to their putative functions generated by BLAST analysis. Expression of five selected genes, Met, SAM, V-ATPase, Cry (Cryptochrome gene), and An (diphthine synthase gene) were examined by RT-PCR and Real-time PCR. Both RT-PCR and Real-time PCR results demonstrated that the differential expressions of these genes were attributable to delaying senescence by exogenously applied gibberellic acid. This is the first genome-wide study of senescence in a species of turfgrass. Zoysiagrass (Zoysia japonica) is one of the important turfgrass species. Extending green period of zoysiagrass via delaying leaf senescence will make this species have more potential in the turfgrass industry. In this study, we found that zoysiagrass seedlings treated with GA3 could delay the leaf senescence induced by darkness. To study expression of genes responsive to staying green in zoysiagrass, suppression subtractive hybridization (SSH) was used to identify differentially expressed genes between non-GA3-treated and GA3-treated seedlings subjected to darkness. A total of 307 ESTs were generated, of which 226 ESTs clustered into 54 contigs and 81 were singlets. Differentially expressed genes selected by subtractions were classified into six categories according to their putative functions generated by BLAST analysis. Expression of five selected genes, Met, SAM, V-ATPase, Cry (Cryptochrome gene), and An (diphthine synthase gene) were examined by RT-PCR and Real-time PCR. Both RT-PCR and Real-time PCR results demonstrated that the differential expressions of these genes were attributable to delaying senescence by exogenously applied gibberellic acid. This is the first genome-wide study of senescence in a species of turfgrass.
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Li, Xue,Cheng, Xiaoxia,Liu, Jun,Zeng, Huiming,Han, Liebao,Tang, Wei The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.1
The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.
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