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- 저자 : Huang Xingrui (검색결과 2 건)
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Huang Xingrui,Yang Jie,Zou Jie,Wen Xi,Wu Tao,Tian Xiangrong,Luo Jiahao,Niu Yahui,Huang Xinglong 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.2
The larvae of Protegira songi Chen & Zhang are notorious defoliator specifically infesting the medicinal plant Eucommia ulmoides Oliver. In insects, Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are highly expressed in olfactory organs and play essential roles in perceiving female pheromone and host volatiles by binding and transporting odorant compounds. In this study, 17 OBPs and 16 CSPs were identified from P. songi by transcriptome analysis. Sequence alignment indicated that PsonOBPs and PsonCSPs share no identity with each other in protein sequence. The overall structures of PsonOBPs and PsonCSPs are mainly constructed by α-helix bundles and loops. But the distribution of conserved cysteine residues and arrangement of α-helix are varied between PsonOBPs and PsonCSPs. Putative ligand binding cavities were found in these predicted protein structures. Expression profiles of PsonOBPs and PsonCSPs in adults and larvae were determined by RT-PCR which showed that 16 PsonOBPs and 11 PsonCSPs were expressed in adult antennae or/and larval head. PsonGOBPs, PsonPBPs, PsonOBP5, PsonOBP7, and PsonCSP6 were preferentially expressed in olfactory associ ated tissues. Our results suggest that the PsonOBPs and PsonCSPs that contain ligand cavity and are highly enriched in olfactory tissues may play roles in chemosensory perception of P. songi, and the transcriptome data may facilitate the studies on the olfactory mechanism of P. songi specifically choosing E. ulmoides as host.
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Yang-Jie Zhao,Huang Xingrui,Wen Fasheng,Huang Xinglong,Liu Zhixiao,Zhang Youxiang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Glutathione S-transferases (GSTs) are multifunctional detoxification enzymes which play important roles in protecting the organisms from environmental stress and are widely used as biomarkers for environmental bio monitoring studies. In this study, We investigated the GST activity in Protohermes costalis larvae, aquatic insects mainly found in oligotrophic fresh water environments, in response to cadmium (Cd) exposure. Eight GST genes in the larvae were identified and their expression patterns under Cd stress were determined by real-time quantitative polymerase chain reaction (RT-qPCR). The GST activity in the whole body was up-regulated by CdCl 2 in a dose-dependent manner and the midgut and malpighian tubules may be the main sites involved in GST activity regulation. The identified PcGSTs are members of cytosolic GST family containing the conserved glutathione-binding domain and substrate-binding domain. Six of them are highly enriched in malpighian tu bules, midgut or/and fat body. The expression level of PcGSTe1 was significantly up-regulated by 0.05, 0.1, 0.5, and 1 mM CdCl 2 treatments when compared with no Cd control. PcGSTs1 expression was significantly higher in 0.05 mM CdCl 2 and lower in 1 mM CdCl 2 compared with no Cd control. Other PcGSTs were up-regulated by different concentrations of CdCl 2 . Our results suggested that P. costalis midgut and malpighian tubules may be the main sites for GST activity regulation that induced by Cd in aqueous phase and increasing expression levels of different PcGST genes may be responsible for the GST activity up-regulation. Moreover, GST activity and gene expression in this insect may be used as biomarkers for future aquatic biomonitoring studies.
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