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Chia-Hung Kuo,Hsin-Yin Teng,Cheng-Kang Lee 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
Glucose dehydrogenase (GDH) locates in thecytoplasmic membrane of Gluconacetobacter xylinus oxidizesglucose to gluconic acid that decreases the conversion ofglucose to bacterial cellulose (BC). In this study, a mutantof G. xylinus was generated by knocking-out the membranebound GDH gene via homologous recombination of adefect GDH gene. The production of BC by G. xylinusmutant (GDH-KO strain) using glucose as a carbon sourcewas investigated. Without the membrane bound GDHactivity, the mutant strain still produces BC and increasesglucose utilization efficiency for cellulose biosynthesis. Incontrast, the wild-type strain oxidized a large fraction ofglucose to gluconic acid that decreased the conversionyield of glucose to BC. Our results showed that the BCproduction from GDH-KO strain was about 40 and 230%higher than that of wild-type strain in static and shakenculture, respectively.