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Surprise, Image and Life: Take the “life lost” event in the Oriental life world as an example
Li Xiaoyun,Wang ping,Hou jialin 아시아사회과학학회 2021 Jornal of Asia Social Science Vol.5 No.3
In the face of images, people s feelings are not necessarily rich, but may also be confused. Whether it is rational or aesthetic. Because emotionally, what they bring is tiredness and boredom. They are narrow-minded and not full. The simple idea of life world is the perfect existence. Even the “life lost”event can also “transform” our spirit. Simple life, not simple life.
Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
Xusheng Dong,Chen Liu,Jialin Miao,Xueyan Lin,Yun Wang,Zhong-Hua Wang,Qiuling Hou 한국축산학회 2022 한국축산학회지 Vol.64 No.5
5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is achemical signal produced by the mammary epithelium cell. In cows, studies have shown that5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammarygland. However, studies in other tissues have shown that 5-HT can effectively promotecell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows isstill unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability ofbovine mammary epithelial cells and its related signaling pathways, (2) interaction betweenprolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T)were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then wereassayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. Theresults suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which wasmainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increasecaused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translationelongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-Tcells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and thelevel of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. Inconclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT.