http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hosseini-Abari, Afrouzossadat,Emtiazi, Giti,Lee, Sang-Hyuk,Kim, Byung-Gee,Kim, June-Hyung Humana Press 2014 Applied biochemistry and biotechnology Vol.174 No.1
<P>Seeking for simple, rapid, and environmental-friendly routes to produce metal nanoparticles is quite attractive for various biotechnological applications. Biological synthesis method of silver nanoparticles has been found very promising due to their non-toxicity and simplicity. Here, the spores of Bacillus stratosphericus isolated from soil enriched with 30 % H2O2 were used for the production of silver nanoparticles. Furthermore, the possible mechanism of silver nanoparticle synthesis by the spores was elucidated for the first time. In this regard, dipicolinic acid (DPA) was shown to play a critical role as a nanoparticle-producing agent. UV-Vis absorption spectroscopy, X-ray diffraction technique, energy-dispersive spectroscopy, and transmission electron microscopy were used to characterize the nanoparticles. Unlike vegetative cells of B. stratosphericus, the spores and the purified DPA were capable of producing nanoparticles from silver nitrate (AgNO3). These biogenic nanoparticles, which were highly toxic against different pathogenic bacteria, showed mixed structures including spherical, triangular, cubic, and hexagonal with the approximate size between 2 and 20 nm in diameter. Our results illustrated the role of dipicolinic acid as a main factor for the synthesis of nanoparticles by the bacterial spores.</P>
Afrouzossadat Hosseini Abari,Maziyar Tayebi 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.3
Orobol (5,7,3',4'-tetrahydroxyisoflavone) is a highly hydroxylated isoflavone, which is rarely found in natural environment. In this study, orobol was produced due to bioconversion of a soybean frequented isoflavone, genistein (4',5,7-Trihydroxyisoflavone) by an active, stable, reusable genetically immobilized enzyme, the recombinant Bacillus subtilis spore displayed tyrosinase. Thin layer chromatography and high performance liquid chromatography were used to monitor the reaction. The results revealed that 1 mM orobol was produced from 3ʹ-hydroxylation of 1 mM genistein by spore displayed tyrosinase at 37oC during 90 min incubation. To study on anti-proliferative effects of orobol, MCF-7 breast cancer cell viability was determined by MTT method and flow cytometric analysis. The comparison between reduction in cell viabilities in 50 to 500 µM genistein and orobol treated cells revealed that orobol has more remarkable anticancer effects than genistein. Flow cytometric analysis showed more than 87% cytotoxicity in 500 µM orobol treated cells by flow cytometric analysis. To the best of our knowledge this is the first report of the orobol demonstrated potent anticancer activity against MCF-7 breast cancer cell. It is suggested that enzymatic biotransformation of soybean genistein to orobol will be made a new approach to create highly bioactive products usable in food and pharmaceutical industries.
Hamidreza Hagh Ranjbar,Afrouzossadat Hosseini Abari,Seyed Mahdi Ghasemi,Najmeh Ghorbani 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4
Epsilon-Poly-L-lysine (ε-PL) is a non-toxic cationic homopolypeptide with unique bioactive properties. In this study, two novel polylysine producers, Paenibacillus polymyxa HS6 and Stenotrophomonas maltophilia YS8 were isolated from different soil samples and identified by 16S rDNA sequence analysis. To obtain pure cationic polypeptide from these bacteria, chemical precipitation with sodium tetraphenylborate was used. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, High-performance liquid chromatography, and Fourier-transform infrared spectroscopy confirmed the purified cationic compound to be polylysine. The antioxidant activity of ε-PL was determined by the 2,2-diphenyl-1-picryl-hydrazyl-hydrate method. Moreover, the anticancer and cytotoxic effects were evaluated against MCF-7, HT-29, and L929 cells by thiazolyl blue tetrazolium bromide and flow cytometry. The maximum yields of 1.8 and 0.39 g/L polylysine were obtained by P. polymyxa HS6 and S. maltophilia YS8, respectively. The results showed that radical scavenging activity of polylysine at a minimum concentration (0.18 mg/mL) was at least 7% which increased to 90% at 8 mg/mL. Furthermore, the highest anticancer activity was observed against the MCF-7 cells (99.5%) at 0.5 mg/mL concentration, while almost no toxicity was observed towards L929 cells. Based on the results obtained in this study, it is suggested that the polylysine produced by P. polymyxa HS6 and S. maltophilia YS8 is a potential bioactive compound with significant anticancer and antioxidant properties.
Wooil Kim,Daeun Kim,Sanggeun Back,Yong-Suk Lee,Afrouzossadat Hosseini Abari,Junehyung Kim 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.2
In this paper, we report removing heavy metal using Bacillus subtilis spore surface display system. We used CotE protein as an anchoring motif because of its high abundance and location in the outer coat layer. And we inserted His12 (double histidine 6 tag) at the C-terminal end of anchoring motif. The proper expression of CotEHis12 fusion protein (22.8 kDa) was confirmed by western blot. We confirmed the surface expression of the CotEHis12 fusion protein using flow cytometry. We tried Ni2+ and Cd2+ adsorption with recombinant spore DB104 (pCotE-His12) and DB104 spore. The amount of adsorbed Ni2+ was 18.2 nmol/mg for DB104 spore and 82.4 nmol/mg for DB104 (pCotE-His12) spore. In the case of Cd2+, the adsorbed amount was 32.6 nmol/mg for DB104 spore and 79.1 nmol/mg for DB104 (pCotE-His12) spore. This means that our spore displayed His12 system can be generally applied for the removal of various kind of heavy metals in the field.