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( Sharafi hakimeh ),( Hadi Maleki ),( Gholamreza Ahmadian ),( Hossein Shahbani Zahiri ),( Neda Sajedinejad ),( Behzad Houshmand ),( Hojatollah Vali ),( Kambiz Akbari Noghabi ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2
Among several bacteria examined, an antibacterial-producing Lactobacillus strain with probiotic characteristics was selected and identified based on 16S rRNA gene sequencing. Subsequent purification and mode of action of the antibacterial compounds on target cells including E. coli were investigated. Maximum production of the antibacterial compound was recorded at 18 h incubation at 30oC. Interestingly, antibacterial activity remained unchanged after heating at 121oC for 45 min, 24 h storage in temperature range of 70oC to room temperature, and 15 min exposure to UV light, and it was stable in the pH of range 2-10. The active compounds were inactivated by proteolytic enzymes, indicating their proteinaceous nature, and, therefore, referred to as bacteriocin-like inhibitory substances. Isolation and partial purification of the effective agent was done by performing ammonium sulfate precipitation and gel filtration chromatography. The molecular mass of the GFC-purified active compound (~3 kDa) was determined by Tris-Tricine SDS-PAGE. To predict the mechanisms of action, transmission electron microscopy (TEM) analysis of ultrathin sections of E. coli before and after antibacterial treatment was carried out. TEM analysis of antibacterial compounds-treated E. coli demonstrated that the completely altered bacteria appear much darker compared with the less altered bacteria, suggesting a change in the cytoplasmic composition. There were also some membrane-bound convoluted structures visible within the completely altered bacteria, which could be attributed to the response of the E. coli to the treatment with the antibacterial compound. According to the in vivo experiments oral administration of L. plantarum HKN01 resulted in recovery of infected BALB/c mice with Salmonella enterica ser. Typhimurium.
Elnaz Khadivinia,Hakimeh Sharafi,Faranak Hadi,Hossein Shahbani Zahiri,Sima Modiri,Azadeh Tohidi,Amir Mousavi,Ali Hatef Salmanian,Kambiz Akbari Noghabi 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
In this study, biosorption of cadmium (II) ions from aqueous solutions by a glyphosate degradingbacterium, Ochrobactrum sp. GDOS, was investigated in batch conditions. The isolate was able to utilize3 mM GP as the sole phosphorous source, favorable to bacterium growth and survival. The effect ofdifferent basic parameters such as initial pH, contact time, initial concentrations of cadmium ion andtemperature on cadmium uptake was evaluated. The adsorption process for Cd (II) is well fitted withLangmuir adsorption isotherm. Experimental data were also tested in terms of biosorption kinetics usingpseudo-first-order and pseudo-second-order kineticmodels. Maximummetal uptake qmax was obtainedas 83.33 mg g1. The sorption process of cadmium onto the Ochrobactrum sp. GDOS biomass followedsecond-order rate kinetic (R2 = 0.9986). A high desorption efficiency was obtained in pH 2. Reusability ofthe biomass was examined under successive biosorption–desorption cycle repeated thrice. Thecharacteristics of the possible interactions between biosorbent and metal ions were also evaluated byscanning electron microscope (SEM), Fourier transform infrared (FT-IR) and X-ray diffraction analysis.