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RISS 인기검색어
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- 저자 : Hongwei Cao (검색결과 4 건)
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Wang, Xiudan,Lin, Hong,Cao, Limin,Zheng, Hongwei,Cui, Mengqi,Du, Shuyuan,Sui, Jianxin 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5
The interaction between some proteins and immune globulin has been confirmed as an important source of matrix interference with the immunoassay of fishery products, but detailed biochemical properties of these proteins have not been indicated. Two interference-inducing proteins (42 and 36 kD) in flounder were isolated, characterized, and identified. Their influences on the immunoassay of norfloxacin were confirmed by western blotting and enzyme-linked immunosorbent assay. The pI value and pH stability of the two proteins were also investigated. Using LC-MS/MS, the two proteins were identified as fructose-bisphosphate aldolase, and such results were partly verified by the aldolase activity of the 42 and 36 kD isolates. Considering the prevalence of these proteins (as multi-functional aldolase of muscles) in foods, these results would help to further understand the matrix effects in various immunoassays as well as the development of effective techniques to improve the efficiency of immunoassays.
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FATIGUE ANALYSIS OF WELD REGION IN TORSION BEAM REAR SUSPENSION SYSTEM
Nan Zhan,Xiaochuan Zhang,Xulong Jin,Hongwei Cao 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.2
Torsion beam rear suspension systems have been widely used in small passenger vehicles. However, welding can lead to local stress concentrations, residual stresses and other various defects, which affects the fatigue life of this system. In this paper, 3D digital image correlation (DIC) technique was used to measure the weld region deformation of a torsion beam rear suspension system in the bench test. The test results show that the obvious strain concentration occurred at the welding seam under the cyclic loading. The numerical simulation work by finite element analysis (FEA) has been done to predict the fatigue damage of the torsion beam rear suspension system. The simulation results show that the location of the fatigue crack was at the strain concentration region in the test work. The enhancement test was performed on a proving ground. The fatigure crack location of the torsion beam rear suspension system in the test automobile was coincident with the results by DIC and FEA. If the load conditions of bench tests are close to the road test, DIC is a useful method to predict the fatigue damage by the strain concentration. As a in lab test it would reduce the cost of fatigue analysis.
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Xiudan Wang,Hong Lin,Limin Cao,Hongwei Zheng,Mengqi Cui,Shuyuan Du,Jianxin Sui 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5
The interaction between some proteins and immune globulin has been confirmed as an important source of matrix interference with the immunoassay of fishery products, but detailed biochemical properties of these proteins have not been indicated. Two interference-inducing proteins (42 and 36 kD) in flounder were isolated, characterized, and identified. Their influences on the immunoassay of norfloxacin were confirmed by western blotting and enzyme-linked immunosorbent assay. The pI value and pH stability of the two proteins were also investigated. Using LC-MS/MS, the two proteins were identified as fructose-bisphosphate aldolase, and such results were partly verified by the aldolase activity of the 42 and 36 kD isolates. Considering the prevalence of these proteins (as multi-functional aldolase of muscles) in foods, these results would help to further understand the matrix effects in various immunoassays as well as the development of effective techniques to improve the efficiency of immunoassays.
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Li Cong,Cai Wentao,Liu Shuli,Zhou Chenghao,Cao Mingyue,Yin Hongwei,Sun Dong-Xiao,Zhang Shengli,Loor Juan J. 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11
Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5’-regulatory region (5’-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5’-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D’ = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.
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