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      저자 : Hongpeng He (검색결과 3 건)
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        Parametric Optimization of Vortex Shedder based on Combination of Gambit, Fluent and iSIGHT

        Nyein, Su Myat,Xu, He,YU, Hongpeng Korean Society for Fluid machinery 2016 International journal of fluid machinery and syste Vol.9 No.2

        In this paper, a new framework that works the automatic execution with less design cycle time and human intervention bottlenecks is introduced to optimize the vortex shedder design by numerical integration method. This framework is based on iSIGHT combined with the pre-processor GAMBIT, and flow analysis software FLUENT. Two vortex shedders, circular with slit and triangular- semi circular cylinder, are employed as the designed models to be optimized, and DOE driver is used for optimization. According to the essential properties of a vortex shedder, it has found that the best diameters are 30mm for circular cylinder with slit and 30 to 35 mm for tri-semi cylinder. For slit ratio, 0.1 and 0.15 are the optimized values for circular with slit and tri-semi cylinder respectively. And it is found that these optimal results generated by DOE automated design cycle are in well agreement with the experiment.

      • KCI등재

        Parametric Optimization of Vortex Shedder based on Combination of Gambit, Fluent and iSIGHT

        Su Myat Nyein,He Xu,Hongpeng YU 한국유체기계학회 2016 International journal of fluid machinery and syste Vol.9 No.2

        In this paper, a new framework that works the automatic execution with less design cycle time and human intervention bottlenecks is introduced to optimize the vortex shedder design by numerical integration method. This framework is based on iSIGHT combined with the pre-processor GAMBIT, and flow analysis software FLUENT. Two vortex shedders, circular with slit and triangular- semi circular cylinder, are employed as the designed models to be optimized, and DOE driver is used for optimization. According to the essential properties of a vortex shedder, it has found that the best diameters are 30 ㎜ for circular cylinder with slit and 30 to 35 ㎜ for tri-semi cylinder. For slit ratio, 0.1 and 0.15 are the optimized values for circular with slit and tri-semi cylinder respectively. And it is found that these optimal results generated by DOE automated design cycle are in well agreement with the experiment.

      • KCI등재

        Tip60 and p300 function antagonistically in the epigenetic regulation of HPV18 E6/E7 genes in cervical cancer HeLa cells

        Yongwei Lai,Zhao He,Aowei Zhang,Zhinan Yan,Xiao Zhang,Shiyue Hu,Nan Wang,Hongpeng He 한국유전학회 2020 Genes & Genomics Vol.42 No.6

        Background High-risk HPV is a causative factor of cervical cancer. HPV DNA fragments integrate into host genome resulting in the constitutive expression of HPV genes E6 and E7 under the regulation of transcription factors, such as p300 and Tip60. Interestingly, Tip60, a factor with HAT (histone acetyl transferase) activity, represses HPV18 E6/E7 genes while another HAT p300 activates the transcription of HPV18 E6/E7. Objective To explore the mechanism for the opposite roles of Tip60 and p300 in the virus gene regulation, and the influence of Tip60 and p300 in histone modifications in the regulatory sequence of HPV18 genes. Methods Tip60 or p300 was either knocked down or overexpressed in HeLa cells. The effects on HPV E6E7 expression were determined with RT-qPCR. The association of RNA polymerase II and the enrichment of acetylated or methylated histones in HPV promoter region were measured by ChIP assays with specific antibodies. Results ChIP results showed that Tip60 and p300 differently affected the modifications of histone H3K9 and the deposition of nucleosomes in HPV18 long control region (LCR). HPV18 LCR in HeLa cells is bivalent chromatin carrying both the active histone H3K9 acetylation mark and the repressive histone H3K9 trimethylation mark, the balance is maintained by Tip60 and p300. Conclusion(s) Based on the roles of Tip60 and p300 in HPV gene regulation, chemical compounds targeting Tip60 or p300 are potential anti-cervical cancer drugs.

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