http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Hexin Tan (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Metabolic Engineering of Vitamin C Production in Arabidops
Ling Xiao,Ying Xiao,Zinan Wang,Hexin Tan,Kexuan Tang,Lei Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Vitamin C (L-ascorbic acid, AsA) is a compound which provides major nutritional value, both for plants and humans. In this study, three distinct metabolic engineering strategies, including overexpression of biosynthesis enzyme, suppression of catabolism enzyme and switching the sub-cellular localization of compartment enzyme, were employed to enhance the production of AsA in Arabidopsis thaliana. The results showed that (1) overexpression of L-Galactose-1-P Phosphatase (GalPPase) enhanced AsA content to 3.96 μmol/g FW, which was 1.6-fold more than that in their wild-type (WT) counterparts; (2) RNAi suppression of ascorbate oxidase (AO) resulted in a significant increase of AsA accumulation (0.86 μmol/g FW) in apoplast; (3) both mitochondrion-target and none-target overexpression of L-Galactose dehydrogenase (GalDH) did not significantly promote AsA production compared with WT (1.96 μmol/g), however a dramatic enhancement was observed following infiltration with L-galactono-1, 4-lactone (L-GalL), both in transgenic and WT plants. The best line produced AsA with the content of 3.90 μmol/g FW, which was about 2-fold of that in the untreated control (1.99 μmol/g FW). This study provides new strategies including GalPPase overexpression, AO suppression as well as L-GalL feeding for modern breeding aimed at stimulating the AsA content in plants.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
